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| Expression and Optimization Strategy of Recombinant Proteins in Chlamydomonas Chloroplast |
| LIN You-hong, CHENG Xia-ying, YAN Yi-wen, LIANG Zong-suo, YANG Zong-qi |
| College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China |
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Abstract In recent years, transgenic technology has developed rapidly, and it has found an increasingly wide utilization in medical and industrial fields. Drug therapy which based on recombinant proteins is the fastest growing technology in pharmaceutical production areas. Their high specificity and low side effect makes treatment efficiency very prominent. However, the complexity of recombinant proteins synthesis also bring production to a certain restrictions. In order to promote the application of these recombinant proteins, expression systems that fit it and strategies that can facilitate it were explored. Compared to other traditional production systems, the research found that Chlamydomonas chloroplast as a bioreactor has several incomparable advantages, it can achieve more rapidly, high yields, lower cost on the production of recombinant proteins, and can be grown in artificial medium and artificially controlled conditions, which reduce the risk of contamination. Through the application of some new techniques, synthesis of recombinant proteins has also been improved. Therefore,Chlamydomonas chloroplast will play a great role in the field of biotechnology in the future as a recombinant proteins with pharmaceutical relevance bioreactor. The expression of recombinant proteins and the optimization strategies in Chlamydomonas chloroplast were reviewed.
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Received: 17 April 2017
Published: 25 October 2017
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