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| Effects of DLX1 on BMP9-induced Osteogenic Differentiation of C3H10T1/2 Mesenchymal Stem Cells |
| XU Li, JI Cai-xia, LIU Xiao-hua, YU Ting-ting, LUO Jin-yong |
| Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
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Abstract Objective:To confirm the effects of DLX1 on BMP9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells.Methods:C3H10T1/2 cells were infected with recombinant adenoviruses expressing BMP9.Expression level of DLX1 upon BMP9 stimulation was measured by RT-PCR and Western blot. Then, the effects of DLX1 on BMP9-induced osteogenic differentiation of C3H10T1/2 cells were determined by ALP staining, Alizarin red S staining, immunocytochemical and entopic bone formation assay. Luciferase reporter assay and Western blot were conducted to analyze the activation level of Smad1/5/8 signal. Results:BMP9 increased the DLX1 expression of C3H10T1/2 cells. BMP9-induced ALP activity, calcium deposition and OCN expression of C3H10T1/2 cells in vitro was enhanced by overexpression of DLX1, but was inhibited by RNAi of DLX1. Overexpression of DLX1 led to an increase in new bone formation compared with the BMP9 group, however, RNAi of DLX1 resulted in a decrease in new bone formation in vivo. Moreover, BMP9-induced activation of Samd1/5/8 were accordingly increased along with overexpression of DLX1, and yet decreased along with DLX1 RNAi. Conclusions:Collectively, these above results implied us that DLX1 may play a private role in regulating BMP9-induced osteogenic differentiation of MSCs at last partly through Smad1/5/8 signal activation.
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Received: 18 December 2016
Published: 25 October 2017
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