Genome Shuffling Enhances Transglutaminase Production of <i>Streptomyces mobaraensis</i>

doi:10.13523/j.cb.20170914

China Biotechnology

2017, Vol. 37

Issue (9): 105-111 DOI: 10.13523/j.cb.20170914

Genome Shuffling Enhances Transglutaminase Production of Streptomyces mobaraensis

SONG Jia-wen¹, TIAN Su¹, ZHANG Yu-ru², WANG Zhi-zhen¹, CHANG Zhong-yi¹, GAO Hong-liang¹, BU Guo-jian², JIN Ming-fei¹

1. School of Life Science, East China Normal University, Shanghai 200241, China;
2. Taixing Dongsheng Food Co. Ltd, Taixing 225411, China

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Abstract Transglutaminase can catalyze Trans-phosphatidylation reaction, and is widely used in food industry. But the production of transglutaminase cannot meet the requirement of industrial production. Nowadays, random mutagenesis is the most frequent but inefficiency method to improve transglutaminase production. Transglutaminase production of Streptomyces mobaraensis is enhanced by genome shuffling. With 96-well plates high-throughput screening, the time and work are reduced. Firstly, protoplasts presentation conditions are optimized. The optimization conditions are treating mycelium by 6mg/ml lysozyme solution for 2 hours. Protoplasts are inactivated by UV illumination for 2 minutes and water-bath in 60℃ for 40min. Then fusants are picked up regenerating in regeneration medium and then screened by 96-well plates high-throughput screening and verified the high-production strains by fermentation in shaking flask. After two rounds of genome shuffling, a strain can produce TGase 65.5% higher than incipient strain is obtained and the production reaches 7.12U/ml.

Key words： Transglutaminase Streptomyces mobaraensis Genome shuffling High-throughput screening

Received: 28 February 2017 Published: 25 September 2017

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	JIN Ming-fei
	SONG Jia-wen
	ZHANG Yu-ru
	CHANG Zhong-yi
	GAO Hong-liang
	BU Guo-jian
	TIAN Su
	WANG Zhi-zhen

Cite this article:

SONG Jia-wen, TIAN Su, ZHANG Yu-ru, WANG Zhi-zhen, CHANG Zhong-yi, GAO Hong-liang, BU Guo-jian, JIN Ming-fei. Genome Shuffling Enhances Transglutaminase Production of Streptomyces mobaraensis. China Biotechnology, 2017, 37(9): 105-111.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170914 OR https://manu60.magtech.com.cn/biotech/Y2017/V37/I9/105

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