Analysis of Antibiotics Quantification Methods

doi:10.13523/j.cb.20160616

China Biotechnology

2016, Vol. 36

Issue (6): 119-126 DOI: 10.13523/j.cb.20160616

Analysis of Antibiotics Quantification Methods

SHEN Wei-tao¹, WANG Ming-yu¹, WANG Yun-kun², WANG Xin-hua², XU Hai¹

1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;
2. School of Environmental Engineering, Shandong University, Jinan 250100, China

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Abstract

Being antibacterial chemicals, antibiotics play a very important role in modern medication. The Chinese pharmacopoeia was revised in 2015 which included 363 antibiotics, and introduced four methods to measure the content of antibiotics. A summary of antibiotics measurement methods showed that among the 363 antibiotics, 275 antibiotics are measured using high performance liquid chromatography, 75 antibiotics are measured using microbial assays, 8 antibiotics are measured using ultraviolet-visible spectrophotometry, and only 5 antibiotics are measured by titration. A summary of methods for the quantification of the content of antibiotics based on the latest Chinese pharmacopoeia revised in 2015 was provided and analyzed, hoping to provide a reference in the determination of the content of residual environmental antibiotic.

Key words： HPLC The Chinese pharmacopoeia Quantification technique Antibiotics

Received: 15 February 2016 Published: 25 June 2016

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Cite this article:

SHEN Wei-tao, WANG Ming-yu, WANG Yun-kun, WANG Xin-hua, XU Hai. Analysis of Antibiotics Quantification Methods. China Biotechnology, 2016, 36(6): 119-126.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160616 OR https://manu60.magtech.com.cn/biotech/Y2016/V36/I6/119

[1] 国家药典委员会.中华人民共和国药典 (二部) .北京:中国医药科技出版社.2015. Chinese Pharmacopoeia Commission.Pharmacopoeia of People's Republic of China(part two).Beijing:China Medical Science Press,2015.
[2] 周启星,罗义,王美娥.抗生素的环境残留、生态毒性及抗性基因污染.生态毒理学报,2007,3:243-251. Zhou Q X,Luo Y,Wang M E.Environmental residues and ecotoxicity of antibiotics and their resistance gene pollution.Asian Journal of Ecotoxicology,2007,3:243-251.
[3] 韩立.高效液相色谱技术在中兽药检验中的应用研究. 河南畜牧兽医(综合版), 2010,3:11-12. Han L.Application of high performance liquid chromatography in the veterinary drug inspection.Henan Journal of Animal Husbandry and Veterinary Medicine,2010,3:11-12.
[4] 颜彦.HPLC-ELSD法测定注射用硫酸异帕米星含量.广东药学院报,2007,03:272-273. Yan Y. Determination of isepamicinsulfate by HPLC-ELSD.Journal of Guangdong Pharmaceutical College,2007,03:272-273.
[5] 国家药典委员会.中华人民共和国药典 (二部) . 北京:中国医药科技出版社,2015.872-873. Chinese Pharmacopoeia Commission.Pharmacopoeia of People's Republic of China(part two).Beijing:China Medical Science Press,2015.872-873.
[6] 刘晔,王洪新,戴军,等.固相萃取-高效液相色谱法测定猪肝中的大环内酯类抗生素.食品与发酵工业,2008,05:162-165. Liu Y,Wang H X,Dai J,et al.Determination of macrolides in pig liver by Solid-phase Extraction coupled with HPLC.Food and Fermentation Industries,2008,05:162-165.
[7] 国家药典委员会.中华人民共和国药典 (二部). 北京:中国医药科技出版社,2015.873. Chinese Pharmacopoeia Commission. Pharmacopoeia of People's Republic of China(part two).Beijing: China Medical Science Press,2015.873.
[9] 月旭科技(上海)股份有限公司.罗红霉素-2015版中国药典项目.http://www.chemalink.net/ChromGall/html/5f9060eac879d243/%C2%DE%BA%EC%C3%B9%CB%D8.html,2015-01-27. YueX Technology (Shanghai), LTD. Roxithromycin-2015 edition of Chinese pharmacopoeia.http://www.chemalink.net/ChromGall/html/5f9060eac879d243/%C2%DE%BA%EC%C3%B9%CB%D8.html,2015-01-27.
[10] 周鲁, 夏昆华. 喹诺酮类化合物的定量构动关系. 华西药学杂志,2006,5:433-435. Zhou L,Xia K H.The quantative structure-pharmacokinetic relationship of quinolones compounds.West China Journal of Pharmaceutical Sciences,2006,5:433-435.
[11] 月旭科技(上海)股份有限公司.左氧氟沙星-2015版中国药典. http://www.chemalink.net/ChromGall/html/3a4ed02b35937126/%D1%F5%B7%FA%C9%B3%D0%C7.html,2015-01-27. YueX Technology (Shanghai),LTD.Levofloxacin-2015 edition of Chinese pharmacopoeia.http://www.chemalink.net/ChromGall/html/3a4ed02b35937126/%D1%F5%B7%FA%C9%B3%D0%C7.html,2015-01-27.
[12] 龚星军.高效液相色谱法同时测定3种头孢类抗生素的含量.中外医学研究,2011,23:141-142. Gong X J.Determination of 3 cephalosporin antibiotics by high performance liquid chromatography at the same time.Chinese and Foreign Medical Research,2011,23:141-142.
[13] 陶辉,李文君,陈卫,等.SPE-HPLC法测定水中4种头孢类抗生素. 中国给水排水,2014,10:96-99. Tao H,Li W J,Chen W,et al.Determination of four cephalosporin antibiotics in water by Solid Phase Extraction and High-performance Liquid Chromatography[J].China Water and Wastewater,2014,10:96-99.
[14] 冯雷, 尹丽珠, 孙文通, 等. 禽畜肉中氯霉素残留量的液质联用分析方法.食品科学,2010,04:243-245. Feng L,Yin l Z,Sun W T,et al.Determination of chloramphenicol residues in meat by HPLC-MS.Food Science.2010,04:243-245.
[15] 国家药典委员会. 中华人民共和国药典 (四部) . 北京: 中国医药科技出版社,2015.160. Chinese Pharmacopoeia Commission.Pharmacopoeia of People's Republic of China(part four).Beijing:China Medical Science Press,2015.160.
[16] 苏润昌, 王建华, 陈少新, 等.抗生素含量微生物检定法影响因素探讨. 天津畜牧兽医,1998,03:13-14. Su R C,Wang J H,Chen S X,et al.Study on the affecting factors of microbial assay.Journal of Animal Husbandry and Veterinary,1998,03:13-14.
[17] 杨亚莉, 胡昌勤. 中国药典2005年版微生物检定法的增修订情况及要点. 中国抗生素杂志, 2005,12:721-726. Yang Y L,Hu C Q.The additional and revised description of the Chinese Pharmacopoeia 2005 on the microbiological assay of antibiotics and the explanatory notes of procedures.The Chinese Journal of Antibiotics,2005,12:721-726.
[18] 彭莺.抗生素含量测定方法在《中国药典》2010年版中的应用比较. 广东化工,2011,06:220-221. Peng Y.The application of the methods for assay of antibiotics in Chinese Pharmacopoeia 2010.Guangdong Chemical Industry,2011,06:220-221.

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