Genetic Transformation of Phytoene Synthase Gene into Ginseng Callus Cells

China Biotechnology

2008, Vol. 28

Issue (2): 90-95 DOI:

Genetic Transformation of Phytoene Synthase Gene into Ginseng Callus Cells

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Abstract

Phytoene synthase is a rate-limiting enzyme in the pathway of carotenoid biosynthesis. This paper is aimed at establishing a transformation of Ginseng callus cells, elevating the nutritive value through encouraging the composition of corresponding carotenoid. Taking Ginseng callus cells as acceptor, PSY gene were transformed into cells via Agrobacterium-mediated. PCR and RT-PCR analysis from the transferred gene plants proved that PSY gene has been transferred into Ginseng callus cells and can be expressed. The content of β-carotene was increased by an average of 26 times. This research established a base of improving and raising the contents of carotenoid in the Ginseng callus cell.

Key words： Agrobacterium tumefaciens PSY gene Ginseng callus cells Genetic transformat

Received: 18 October 2007 Published: 25 February 2008

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Cite this article:

. Genetic Transformation of Phytoene Synthase Gene into Ginseng Callus Cells. China Biotechnology, 2008, 28(2): 90-95.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I2/90

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