Inhibition of CaMKIIβ Expression by RNA Interference in PC12

China Biotechnology

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Abstract To investigate the inhibitory effects of RNAi expression vector on CaMKIIβ expression of PC12 cell lines. siRNA expression vectors were constructed to be targeted directly at CaMKIIβ gene. The recombinants were transfected into PC12 cell line with liposome. The expression of CaMKIIβ was detected by RT-PCR and Western blot. Expression vectors could reduce the expressions of CaMKIIβ mRNA and protein in PC12, Compared with blank vector group, the former, the ratio of inhibition of the expression of CaMKIIβ mRNA was 46.40% and 64.69%, in 48th hour and 72th hour, the ratio of inhibitory of the expression of CaMKIIβ protein was 74.77% in 72th hour. RNAi expression vectors can effectively inhibit the expression of CaMKIIβ in PC12.

Key words： shRNA Gene expression CaMKII RNAi

Received: 12 November 2007 Published: 25 February 2008

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. Inhibition of CaMKIIβ Expression by RNA Interference in PC12. China Biotechnology, 2008, 28(2): 76-79.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I2/76

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