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| Study of Rapid Determination for Glycerol Content by Enzyme Electrode |
| SI Hong-yu1,2, WANG Bing-lian3,4, LIANG Xiao-hui1,2, ZHANG Xiao-dong1,2 |
1. Energy Research Institute of Shandong Academy of Sciences, Jinan 250014, China;
2. Key Laboratory for Biomass Gasification Technology of Shandong Province, Jinan 250014, China;
3. Biology Institute of Shandong Academy of Sciences, Jinan, 250014, China;
4. Key Laboratory for Biosensors of Shandong Province, Jinan 250014, China |
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Abstract Glycerokinase (GK) and glycerol-3-phosphate oxidase (GPO) were immobilized to develop glycerol enzyme membrane and enzyme electrode for determination of glycerol concentration. Results showed that the higher current output for glycerol was obtained using a 1: 1 (GK: GPO) ratio for the random immobilization. The efficient two-enzymatic membrane was prepared by immobilizing GK in nuclear pore film coupled with GPO on Biodyne B. Glycerol enzyme electrodes were realized by assembling the GK-GPO membranes previously prepared. In this way, the optimum pH was 7.0, and the fit range of temperature was 28~32℃. In the best experimental conditions, the linear scope was 0.05~9.00 g/L with excellent linearity and high stability. The recovery was 98.4%~102.4%, and its relative standard deviation (RSD) was <5%. There was no significant difference (P>0.05) in measurement results among glycerol enzyme electrode, Periodate oxidation method and HPLC.
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Received: 04 August 2016
Published: 25 December 2016
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