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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (8): 105-111    DOI: 10.13523/j.cb.20140816
    
Recent Developments in Spore Surface Display of Bacillus subtilis
XIE Zhi-dan1, FAN Wen1, JIA Dong-cheng1, YANG Na1, XIA Zheng-yuan2, QIAO Min1
1. Key Laboratory for Conservation and Utilization of Bio-Resource, Yunnan University, Kunming 650091, China;
2. Yunnan Tobacco Research Institute of Agricultural Science, Kunming 650091, China
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Abstract  

Bacillus subtilis spore display, a technique that presents proteins or peptides on the surface of the spore of Bacillus subtilis, enables high-throughput screening and has become an essential tool in bimolecular engineering. The major advantage of spores over the other published systems is their synthesis within the cytoplasm of the bacterial cell. Therefore, any heterologous protein to be anchored on the outside does not have to cross any membrane. Furthermore,spores are extremely resistant against high temperature, irradiation and many chemicals, and can be stored for many years at room temperature. With the advantage of complete genome information and security, Bacillus subtilis spore display now is attracting more and more attention. The following describes the recent progress of surface of Bacillus subtilis in the produce of vaccines and the immobillzed enzyme, and present a brief overview about how to improve the yield of the target protein surface display.

Key words： Surface display      Bacillus subtilis      Antigen      Anchoring protein     
Received: 23 May 2014      Published: 25 August 2014
ZTFLH:  Q819  
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Cite this article:

XIE Zhi-dan, FAN Wen, JIA Dong-cheng, YANG Na, XIA Zheng-yuan, QIAO Min. Recent Developments in Spore Surface Display of Bacillus subtilis. China Biotechnology, 2014, 34(8): 105-111.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140816     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I8/105


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