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Cloning and Expression Analysis One Zinc Finger Protein Gene FtLOL1 in Fagopyrum tataricum: Effect of Abiotic Stress |
GAO Fei, ZHOU Jing, LIU Xiao-tong, LI Cheng-lei, YAO Hui-peng, ZHAO Hai-xia, WU Qi |
College of Life Science, Sichuan Agricultural University, Ya'an 625014, China |
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Abstract According to transcriptome data of Fagopyrum tataricum at flowering, using PCR and RT-PCR techniques, the DNA and cDNA sequences of FtLOL1 (GenBank accession number: KP260662) gene were amplified from Fagopyrum tataricum. The obtained sequences were analyzed by bioinformatics software, and the expression FtLOL1 gene were analysed by qPCR under UV-B, SA and 4℃ cold stress. The results showed that the DNA sequence of FtLOL1 gene was 1 720bp, of which consisted 5 exons and 4 introns, in line with the principle of GT-AG splicing, and the cDNA of FtLOL1 contained a 444bp ORF, encoding 147 amino acids, with the typical features of LSD1 family. The 2mmol/L SA, UV-B radiation and 4℃ cold stress could lead to a significant discrease in the expression of the FtLOL1 gene. The results expected to lay a foundation for the stress resistance study in Fagopyrum tataricum.
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Received: 11 March 2015
Published: 25 August 2015
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