HPT Protein Detection and Expression Pattern in Transgenic Rice

doi:10.13523/j.cb.20150610

China Biotechnology

2015, Vol. 35

Issue (6): 61-67 DOI: 10.13523/j.cb.20150610

HPT Protein Detection and Expression Pattern in Transgenic Rice

YANG Shuo¹, HAO Yu-jie², LAN Jin-ping¹, WEI Han-fu², WEI Jian¹, RONG Rui-juan¹, WU Peng-cheng², LIU Guo-zhen¹, YIN Chang-cheng², LI Li-yun¹

1. College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China;
2. Beijing Protein Innovation Co., Ltd. Beijing 101318, China

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Abstract

Hpt as one of the most commonly selective marker gene was used in transgenic plants. To establish immunoblotting method for detectting HPT protein expression profiles in transgenic plants has important theoretical significance and application value. HPT recombinant protein was expressed in E. coli, and used to prepare specific monoclonal antibody. Western blot was used to detect the expression of HPT protein in transgenic rice of 4021, the results showed that in 10% of single grain of rice (about 1.5mg) contained HPT protein can be detected. Quantitative analysis revealed that the content of HPT protein in rice seedling account for about 0.01 ‰ of fresh weight. The expression profiles of HPT protein under CaMV 35S promoter-driven start in transgenic rice showed that the abundance of HPT was regulated higher levels in the leaves of the seedling and adult, showing a rising trend in the seed filling period, but HPT showing low abundance in the stems, tillers, node, shears, cushion, roots and other tissues. In short, an applicable immunoblot method for detectting HPT protein was established, while the HPT protein expression profiles were analyzed in transgenic rice.

Key words： Transgenic rice HPT protein Western blot Selective marker gene

Received: 18 March 2015 Published: 25 June 2015

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Cite this article:

YANG Shuo, HAO Yu-jie, LAN Jin-ping, WEI Han-fu, WEI Jian, RONG Rui-juan, WU Peng-cheng, LIU Guo-zhen, YIN Chang-cheng, LI Li-yun. HPT Protein Detection and Expression Pattern in Transgenic Rice. China Biotechnology, 2015, 35(6): 61-67.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150610 OR https://manu60.magtech.com.cn/biotech/Y2015/V35/I6/61

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