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| Expression,Purification,Crystallization of SP0306 Protein from Streptococcus pneumoniae |
| HUANG Jian1, HUANG Mei-rong2, ZHU Jie-hua1, LUO Shi-lu1, MIN Xun1 |
1. Affiliated Hospital of Zunyi Medical College, Department of Laboratory Medicine, Zunyi 563003, China;
2. Affiliated Hospital of Zunyi Medical College, Department of Blood Transfusion, Zunyi 563003, China |
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Abstract SP0306 protein is a hypothetical transcription factor of Streptococcus pneumoniae TIGR4 strains, but the protein three-dimensional structure and biological function is unclear. The bioinformatics analyses show that it may regulate the expression of carbohydrate metabolism related genes. The protein expression vector of PET28a-sp0306 was successfully constructed and a soluble form of SP0306 protein was acquired in Escherichia coli BL21 (DE3) strain. High purity SP0306 protein was obtained after Ni-NTA affinity chromatography and DEAE negative ion exchange chromatography. High quality crystal of SP0306 protein was obtained by the vapor diffusion method, then crystal X-ray diffraction was executed.The results provide a basis for helping resolve the structure of SP0306 and further function research.
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Received: 05 March 2015
Published: 25 June 2015
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