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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (04): 89-94    DOI:
    
Screening Human Transferrin Binding Peptides by Phage Display Technology
LU Yan,SHI Yi-Feng
School of Biological & Food Engineering, Dalian Polytechnic University, Dalian 116034, China
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Abstract  

Phage display technology was used to select transferrin binding peptides (TfB) from a phage fused random heptapeptide library. After three rounds of biopanning, positive recombinant phage clones were enriched. For estimation the efficiency of phage display screening the binding peptides, the selection criteria of recovery yield and specificity ratio in the panning process were developed. The affinity constants of the four most positive TfB clones were assayed with the method of the measurement of phage titres, and the corresponding TfB sequences were obtained through DNA sequencing. Because of transferrin receptor as a tumor biomarker, transferrin has been used as a drug carrier to deliver therapeutics targeting cancer cells. Therefore TfBs will be explored as useful protein tags for binding Tf to improve the transport properties of recombinant protein drugs in vivo.



Key wordsPhage display technology      Transferrin      Transferrin binding peptides      Affinity constant      Protein tag     
Received: 21 December 2009      Published: 29 April 2010
Corresponding Authors: Yi-Feng Shi     E-mail: shiyf@dlpu.edu.cn;
Cite this article:

. Screening Human Transferrin Binding Peptides by Phage Display Technology. China Biotechnology, 2010, 30(04): 89-94.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I04/89

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