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| An Efficient Fungal Protein Extraction Method |
| HU Bin-bin, LIN Lian-bing, WEI Yun-lin, JI Xiu-ling, ZHANG Qi |
| School of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China |
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Abstract Protein extraction is the first step of proteomics research. Its quality directly affects the protein expression analysis studies and the accuracy of the results. In order to extract total cellular protein from Mortierella isabellina strain M6-22 for further proteomics and some other related research, five protein extraction methods were tried. Protein concentration and SDS-PAGE analysis of protein abundancy were used to evaluate the effects of these methods, and one of them was selected and modified, by which, a total protein concentration of 2.99 mg/ml was obtained. The modified method had advantage of simpler procedure than the original one and could obtain higher protein abundancy. The modified method was further used to extract the total protein of five other fungi, and the results showed that similar extraction effect could be obtained, indicating that this method can be potentially used as an efficient total protein extraction method for fungi.
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Received: 19 July 2013
Published: 25 September 2013
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