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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2013, Vol. 33 Issue (6): 131-137    DOI:
    
Molecular Detection and Genetic Analysis of Chinese Cabbage Progeny with the IL-4
JIN Xiao-xia, GAO Ya, YU Li-jie
College of Life Science and Technology, Harbin Normal University, Harbin 150025, China
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Abstract  

IL-4 gene transformation mediated by pollen-tube pathway method of Chinese cabbage for molecular detection of descendants using PPT resistance screening, PCR amplification, PCR-Southern hybrid, ELISA, Western hybrid methods, combined with statistical analysis study IL-4 inheritance of genes in transgenic Chinese cabbage in future generations. Results indicated that: (1) PPT resistance in transgenic Chinese cabbage, PPT resistance positive rates of T4 generation was 33.43%, the positive rate of PCR amplification of IL-4 gene was 1.39%. IL-4 separation plant does not meet Mendel’s law of segregation. (2) ELISA test on 14 ones of PCR-positive strains of 5 strains in the T4 generation, 7 samples of which are positive, IL-4 expression was about 326.87~1233.13 ng/g FW, the ones of IL-4 expression within the same strains significance of difference. (3) Western confirmed IL-4 in transgenic Chinese cabbage hybrid offspring in the regular expression. It indicated that IL-4 gene has been incorporated into the Chinese cabbage in the genome with PCR-southern hybrid, and that gene still exists in transgenic plants’ future generations.

Key words： Pollen-tube pathway method      Chinese cabbage      Interleukin-4 (IL-4)      Non-Mendelian Inheritance     
Received: 19 February 2013      Published: 25 June 2013
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JIN Xiao-xia
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JIN Xiao-xia, GAO Ya, YU Li-jie. Molecular Detection and Genetic Analysis of Chinese Cabbage Progeny with the IL-4. China Biotechnology, 2013, 33(6): 131-137.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2013/V33/I6/131

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