| TECHNIQUES AND METHODS |
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| A Novel Method for Constructing Small Fragment Gene of Expression Vector |
| JIN Lei1,2, ZHAO Wen-xiu2, MA Lan2 |
1. School of Life Sciences, Tsinghua University, Beijing 100084, China;
2. Division of Life Science & Health, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China |
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Abstract A new method for rapid constructing small fragment of expression vector is introduced. This method eliminates the need for the target gene digested with restriction enzyme and recovery steps, and eliminates the vector gel purified steps after digestion. It is simple and fast, saving reagent costs, high connection efficiency. Four expression vectors of small fragment gene(67bp) have been completed with this method.
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Received: 07 February 2012
Published: 25 June 2012
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