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| Effects of Different Nitrogen Nutrition Level on the Growth and Photosynthetic Physiology of Odontella aurita |
| WANG Lu-yao, SANG Min, LI Ai-fen, ZHANG Cheng-wu |
| Institute of Hydrobiology, Jinan University, Guangzhou 510632, China |
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Abstract In order to analysis the effects of different nitrogen nutrition level on the growth and photosynthetic physiology of Odontella aurita, diatoms were cultured under different nitrogen conditions(17.6 mmol/L N, 8.8mmol/L N, 5.87 mmol/L N, 0 mmol/L N), using 6cm columnar light biological reactor. The results showed that the algae of different nitrogen groups reached the maximum growth at different time. In the early of culturing, nitrogen limitation(5.87mmol/L N, 8.8mmol/L N) promoted the growth of Odontella aurita comparing with the control, while nitrogen starvation significantly inhibited the growth of the algae(P<0.05).The carbohydrate content increased significantly (P<0.05) under nitrogen limitation conditon, while total protein content decreased significantly (P<0.05).The content of chlorophyll a, c, total carotenoids of Odontella aurit are positively related with nitrogen nutrition levels of culture medium. The maximum photosynthetic oxygen release rate Pm decreased with nitrogen concentration, the respiratory rate of Rd presented the opposite tendency. The maximum efficiency of light energy conversion of PSⅡ (Fv/Fm), the actual energy conversion efficiency(Yield), potential activity(Fv/Fo) and relative electron transfer efficiency (ETR) decreased significantly with the nitrogen limitation. Illuminating that algal cells of apparent photosynthetic physiological status are directly related to the level of nitrogen nutrition.
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Received: 30 December 2011
Published: 25 June 2012
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