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| Biotransformation of Androst-4-en-3,17-dione by Gibberella intermedia C2 |
| YAO Ren-hui1, DONG Zhuo1, LI Hui2 |
1. Tangshan Vocational & Technical College, Tangshan 063000, China;
2. School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China |
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Abstract Steroids with unique biological activity are widely used as anti-inflammatory, diuretic, anti-androgenic, contraceptive and anti-cancer agents. In recent years, the role of biocatalysis and biotransformation in the synthesis of steroid drug intermediates is becoming more and more significant. In order to synthesize the potential steroidal compounds, biotransformation of androst-4-en-3,17-dione (androstenedione, AD) by Gibberella intermedia C2 was investigated. The two products was identified as 15α-OH-AD and 11α,15α-diOH-AD by structure identification. The results of the conversion mechanism research showed that androstenedione is first converted into 15α-OH-AD with the 15α-hydroxylation of AD, and subsequently, into 11α,15α-diOH-AD after the 11α-hydroxylation of 15α-OH-AD. G. intermedia C2 can selectively and sequentially perform two DHEA hydroxylation reactions. In addition, the optimum biotransformation conditions of androstenedione were obtained as follows:initial pH value 6.5, the strain incubated in 250ml shake flask with 30ml medium at 28℃, substrate concentration 6.0g/L, culture for 24h and biotransformation for 84h at 220r/min. The molar conversion reached 81.5% under above conditions.
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Received: 11 October 2016
Published: 25 March 2017
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