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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (6): 107-118    DOI: 10.13523/j.cb.20160615
    
Study Progress and Prospect on γ-poly Glutamic Acid Genetic Engineering
JI Mei-ping, PANG Yan-bo, FU Li-li, NA Ri, GUO Jiu-feng, WANG Zhi-yong
Physical Science and Technology, Inner Mongolia University, Key Laboratory of Ion Beam Bio-engineering in Autonomous Region, Huhhot 010021, China
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Abstract  

γ-poly-glutamic acid (γ-PGA) is a natural,biodegradable,new anionic polymer and synthesized by some bacillus and archaea and one eukaryote.They are divided into glutamate-dependent and non-dependent glutamate.The gene required for γ-PGA contain capsule and polyglutamate synthase.The synthesize organized by the responding expressed protein regulates the synthesis and transport of γ-PGA.γ-PGA is friendly to environment with characteristics of good water solubility, moisture resistance, water absorption, good biological compatibility and biodegradable, edible and environmental pollution, etc..Therefore,γ-PGA and its derivatives have been of interest in a broad range of application prospects such as medicine, agriculture, food, environment, cosmetics. The structural characteristics of γ-PGA, the microbial synthesis related genes, synthesis mechanism, application, mutagenic treatment were focused on.Using technology of physics and chemistry to mutagenize can achieve high-yielding strains of γ-PGA,which provide the basis for improving production of γ-PGA.



Key wordsGene      Mutagenesis      γ-PGA     
Received: 30 November 2015      Published: 25 June 2016
ZTFLH:  Q819  
Cite this article:

JI Mei-ping, PANG Yan-bo, FU Li-li, NA Ri, GUO Jiu-feng, WANG Zhi-yong. Study Progress and Prospect on γ-poly Glutamic Acid Genetic Engineering. China Biotechnology, 2016, 36(6): 107-118.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160615     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I6/107

[1] Ivanovics G B.Chemische and immunologische studien uber mechanimus der milzbrandinfektion and immunitat;die chemische strukt under kapdelsubstanz des milzbrandbasillus undder serologisch identischen spezifischen substanzdes Bacillus mesentericus. Immunitatsforsch, 1937, 90:304-318.
[2] Bovarnick M J.The formation of extracellular D-ghrtamic acid polypeptide by Bacillus subtilis.J Biol Chem,1942,145(7):415-442.
[3] 鞠蕾,马霞,徐弇豪.γ-聚谷氨酸提取条件的优化.食品工业科技,2012,33(4):299-303. Ju L,Ma X,Xu Y H.Optimization of γ-polyglutamic acid extraction condition.Science and Technology of Food Industry,2012,33(4):299-303.
[4] Ashiuchi M, Kamei Misono H. Poly-gamma-glutamate synthesis of Bacillus subtilis.Journal of Molecular Catalysis B-Enzymatic,2003,23(2-6):101-106.
[5] Rao M V,Atreyi M, Chauhan V S,et al. Solution conformation of poly(L-lysyl-L-glutamic acid) and poly(L-lysyl-L-glutamine). Int J Pept Protein Res,1984,24(1):48-54.
[6] 杨革,陈坚,曲音波,等.细菌聚γ-谷氨酸表征的研究.高分子材料科学与工程,2002,18(4):133-136. Yang G,Chen J,Q Y B, et al.Studies on the characterization of bacterial γ-poly(glutamic acid).Polymer Materials Science and Engineering,2002,18(4):133-136.
[7] Borbely M, Nagasaki Y, Borbely J, et al. Biosynthesis and chemical modification of poly(y-glutamic acid). Polymer Bull, 1994, 32(3):127-132.
[8] Crescenzi V D, Alagni M, Dentini M, et al. Aqueous solution properties of bacterial poly-D-glutamate. ACS Symp Ser, 1996, 627(4):233-242.
[9] Goto A, Kunioka M. Biosynthesis and hydrolysis of poly (γ-glumatic acid) from Bacillus subtilis IF03335.Biosci Biotec Biochem,1992,63(1):110-115.
[10] Negus D, Burn J, Sweed A, et al. Poly-d-glutatin is acid capsule interferes with lytic infection of Bacillus anthrariss by B.Anthracis specific bacteriophages.Applied and Environmental Microbiology, 2013, 79 (2):714-717.
[11] Hezayen F F, Rehmb H A, Eberhardt R, et al. Polymer production by two newly isolated extremely halophilic archaea:application of a novel corrosion-resistant bioreactor.Applied Microbiology Biotechnology,2000,54(3):319-325.
[12] Weber J.Poly(Glutamic acid) S are the major constituents of nematecvsts in hydra (hydrozoa, cnidaria).Journal of Biological Chemistry,1990,265(17):9664-9669.
[13] Kocianova S,Vuong C,Yao Y F, et al. Key role of poly-gamma-D-glutamic acid in immune evasion and virulence of Staphylococcus epidermidis.Journal of Clinical lnvestigation,2005,115(3):688-694.
[14] 王浩,杨丽萍,乔君.γ-聚谷氨酸的研究进展.山东食品发酵,2011,163(4):30-34. Wang H,Yang L P, Qiao J.Study of Poly-γ-glutamic acid.Shandong Food Fermentution.2011,163(4):30-34.
[15] Jeong J H, Kim J N, Wee Y J, et al. The statistically optimized production of poly (γ-glutamic acid) by batch fermentation of a newly isolated Bacillus subtilis RKY3. Bioresource Technology,2010,101(12):4533-4539.
[16] 刘晓鸥,李睿颖,徐勇虎,等.聚谷氨酸的生物合成及应用前景.食品工程,2009,3(1):23-27. Liu X O,Li R Y,Xu Y H, et al.Research advances of biosynthes is of poly-γ-glutamic acid and applications and prospect.Food Engineering,2009,3(1):23-27.
[17] Goto A,Kunioka M.Biosynthesis and hydrolysis of Poly(γ-Glutamic Acid) from Bacillus subtilis IFO3335.Bioscience Bio-technology and Biochemistry,1992,63(1):110-115.
[18] Hidetoshi K,Toshio M,Kazumichi U,et al.Production of poly (γ-glutamic acid) by Bacillus subtilis F-2-01.Bioscience Biotechnology and Biochemistey,1993,57(7):1212-1213.
[19] Yoon S H, Do J H, Lee S Y, et al. Production of poly-γ-glutamic acid by fed-batch culture of Bacillus licheformis. Biotechnology Letters, 2000,22(7):585-588.
[20] Gardner J M,Troy F A.Chemistry and biosyn-thesis of the poly (gamma-D-glutamyl) capsule in Bacillus lichenformrs.Activation,racemization and polymerization of glutamic acid by a membranous polyglutamyl synthetase complex.The Journal of Biological Chemistry,1979, 254(14):6262-6269.
[21] Ashiuchi M,Kamei T,Baek D H,et al.Isolation of Bacillus subtilis (chungkookjang),a poly-γ-glutamate producer with high genetic competence.Applied Microbiology and Biotechnology, 2001,57(5-6):764-769.
[22] 杨革,陈坚,曲音波,等.C源和Mn2+对地衣芽孢杆菌WBL-3生产γ-谷氨酸的影响.化工学报,2002,53(3):317-320. Yang G,Chen J,Qu Y B,et al.Effects of carbon source and manganese ion on production γ-poly(glutamate) by Bacillus licheniformis WBL-3.Journal of Chemical Industry and Engineering,2002,53(3):317-320.
[23] 张业伟,魏雪团,胡中波,等.地衣芽孢杆菌P-104发酵生产γ-聚谷氨酸条件优化.过程工程学报,2012,12(2):288-292. Zang Y W,Wei X T,Hu Z B, et al.Optimization of γ-polyglutamic acid production by Bacillus licheniformis P-104.The Chinese Journal of Process Engineering,2012,12(2):288-292.
[24] Ito Y, Tanaka T, Ohmachi T, et al. Glutamic acid independent production of poly (γ-glutamic acid) by Bacillus subtilis TAM-4. Bioscience Biotechnology Biochemistry,1996, 60(8):1239-1242.
[25] Cheng C,AsadaY,Aida T.Production of γ-polyglutamic acid by Bacillus licheniformis A35 under denitrifying conditions.Agricultural and Biological Chemistry,1989,53(9):2369-2375.
[26] Ogawa Y,Yamaguchi F,Yuasa K,et al.Effi-cient production of γ-polyglutamic acid by Bacillus subtilis (natto) in jar fermenters.Biosci Biotechnol Biochem,1997,61:110-115.
[27] 李文倩.γ-聚谷氨酸发酵及提取工艺研究.济南:山东轻工业学院,2010. Li W Q.Study on the fermentation and extraction technology of poly-γ-glutamic acid.Jinan:Shandong Polytechnic University,2010.
[28] 何剑,雍晓雨,周俊,等.一株γ-多聚谷氨酸生产菌的分离筛选与鉴定.生物加工过程,2014,12(4):87-93. He J,Yong X Y,Zhou J,et al.Isolation and identification of Bacillus strain producing γ-polyglutamic acid.Chinese Journal of Bioprocess Engineering,2014,12(4):87-93.
[29] Ashiuchi M,Shimanouchi K,Nakamura H, et al.Enzymatic synthesis of high-molecular-mass poly-gamma-glutamate and regulation of its stereochemistry.Applied and Environment Microbiology,2004,70(7):4249-4255.
[30] Eveland S S,Pompliano D L,Anderson M S.Conditionally lethal Escherchia coli murein contain point defects that map to regions conserved among mureinand folyl poly-gamma-glutamate ligases.Identification of a ligase superfamily.Biochemistry,1997,36(20):6223-6229.
[31] Urushibata Y,Tokuyama S,Tahara Y. Characterization of the Bacillus subtili.s ywsC gene involved in gamma-polyglutamic acid production.Journal of Bacteriology,2002,184 (2):337-343.
[32] Ashiuchi M,Kamei T,Misono H.Poly-gamma-glutamate synthetase complex of Bacillus subtilis.Journal of Molecular Catalysis B-Enzymatic,2003,23 (2-6):101-106.
[33] 姚文娟,范文俊,许小乐,等.γ-聚谷氨酸合成酶系PgsBCA结构的生物信息学分析.南通大学学报(自然科学版),2012,11(2):41-46. Yao W J, Fan W J,Xu X L,et al.Sequence analysis of PgsBCA of poly-γ-glutamate synthetase system.Journal of Nantong University (Natural Science Edition),2012,11(2):41-46.
[34] Mailer U,Antelmann H, Buder T, et al. Bacillus subtilis functional genomics:genome-wide analysis of the DegS-DegC regulon by transcriptomics and protiomics.Molecular Genetics and Genomics,2002,268(4):455-467.
[35] Stanley N R,Lazazzera B A.Defining the genetic differences between wild and domestic strains of Bacillus subtilis that affect poly-gamma-DL-glutamic acid production and biofilm formation.Molecular Microbiology,2005,57(4):1143-1158.
[36] Kimura K,TranL S P,Do T H,et al.Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis(natto).Bioscience Biotechnology and Biochemistry,2009,73(5):1149-1155.
[37] Ashiuchi M,Tani K,Soda K,et al.Properties of glutamate racemase from Bacillus subtilis IFO3336 producing poly-gamma-glutamate.Journal of Biochemistry,1998,123(6):1156-1163.
[38] 吴群,徐虹,许琳.Bacillus subtilis NX-2合成γ-聚谷氨酸的立体构型调控机理.过程工程学报,2006,6(3):458-461. Wu Q,Xu H,Xu L.Regulation of stereochemical composition of poly-γ-glutamic Acid in Bacillus subtilis NX-2.The Chinese Journal of Process Engineering,2006,6(3):458-461.
[39] Troy F A. Chemistry and hiesynthesis of poly (gamma-d-glutamyl) capsule in Bacillus licherrifornais properties of membrane-mediated biosynthetic reaction.Journal of Biological Chemistry,1973,248(1):305-315.
[40] Ashiuchi M,Nawa C,Kamei T,et al.Physilogical and biochemical characteristics of poly gamma-glutamate synthetase complex of Bacillus subtilis.European Journal of Bio-chemistry,2001,268(20):5321-5328.
[41] 郑重,吴剑光,邱乐泉.微生物聚谷氨酸(γ-PGA)合成酶及合成机理的研究进展.生物技术通报,2010,6:52-56. Zheng Z,Wu J G,Qiu L Q, et al.Study progress on poly-γ-glutamate synthetase and synthesis mechanism.Biotechnology Bulletin,2010(6):52-56.
[42] Candela T,Fouet A.Poly-gamma-glutamate in bacteria.Molecular Microbiology,2006,60 (5):1091-1098.
[43] Makino S,Uchida I, Terakado N.Molecular characterization and protein analysis of the cap region,which is essential for encapsulation in Bacillits anthraci.Journal of Bacteriology,1989,171(2):722-730.
[44] Unrushibata Y,Tokttvatn A S,Tahara Y.Difference in tanscription levels of cap genes for γ-poly glutatmic acid production between Bacillus subtilis IFO 16449 and Marburg 168.Journal of Bioscience and Bioengineering, 2002,93(2):252-254.
[45] Hara T,Nagatomo S,Ogata S,et al.The DNA sequence of γ-glutamyl transpeptidase gene of Bacillus subtilis (natto) plasmid pUH1. Applied Microbiology and Biotechnology, 1992,37(2):211-215.
[46] Nagai T,Koguchi K,Itoh Y.Chemical analysis of poly-γ-glutamic acid produced by plasmid-free Bacillus subtilis (natto): evidence that plasmids are not involved in poly-γ-glutamic acid producton. The Journal of General and Applied Microbiology,1997, 43(3):139-143.
[47] Ashiuchi M,Soda K,Misono H.A poly-γ-gltttamate synthetic system of Bacillus sutbtilis IFO 3336:gene cloning and biochemical analysis of poly-γ-glutatmate produced by Escherichia coli clone cells.Biochemical Biophysical Research Communications,1999, 263(1):6-12.
[48] Ashiuchi M,Nawa C,Kamei T,et al. Physiological and biochemicalcharacteristics of poly gamma-glutamate synthetase complex of Bacillus subtilis.European Journal of Biochemistry,2001,268(20):5321-5328.
[49] Xu Q,Sudek S,McMullan D,et al.Strctural basis of murein peptide specificity of a γ-D-glutamyl-L-diatninoacid endopeptidase.Structure,2009,17(2):303-313.
[50] Urutshibata Y,Tokttvatn A S,Tahara Y.Characterization of the Bacillus subtilis ywsC gene, involved in γ-polyglutamic acid production.Journal of Bacteriology,2002,184(2):337-343.
[51] 石峰.微生物制备γ聚谷氨酸的研究.杭州:浙江大学,2006. Shi F.Study on production of poly γglutamic acid by microoganism.Hangzhou:Zhejiang University,2006.
[52] 马婕,王丹,李强,等.基因工程大肠杆菌合成γ-聚谷氨酸.过程工程学报,2009,9 (4):792-795. Ma J,Wang D,Li Q,et al.Biosynthesis of Poly-γ-glutamate acid by Escherichia coli.The Chinese Journal of Process Engineering,2009,9 (4):792-795.
[53] 金映虹,刘静,刘莉,等.利用Bacillus licheniformis NK-03合成聚谷氨酸及其合成酶基因pgsBCA的克隆.南开大学学报(自然科学版),2008,41(3):57-63. Jin Y H,Liu J,Liu L,et al.Production of poly (γ-glutamic acid)by Bacillus licheniformis NK-03 and cloning of γ-PGA biosynthesis genes.Acta Scientiarum Naturalium Universitatis Nankaiensis,2008,41(3):57-63.
[54] 曹名锋,金映虹,解慧,等.γ-聚谷氨酸的微生物合成、相关基因及应用展望.微生物学通报,2011,38(3):388-395. Cao M F,Jin Y H,Xie H,et al.Biosynthesis of poly(γ-glutamic acid),its related genes and application prospects.Microbiology China,2011,38(3):388-395.
[55] 冯志彬,程仕伟,缪静,等.γ-聚谷氨酸生产菌的选育及培养条件研究.生物加工过程,2010,8(1):40-44. Feng Z B, Cheng S W, Miao J,et al.Screening and optimizing culture conditions of Bacillus strain for producing γ-polyglutamic acid.Chinese Journal of Bioprocess Engineering,2010,8(1):40-44.
[56] 索晨,梅乐和,黄俊,等.60Coγ射线诱变选育聚谷氨酸高产菌株及培养基初步优化.高校化学工程学报,2007,21(5):820-825. Suo C,Mei L H,Huang J,et al.Selection of γ-poly glutamic acid high yield strain by 60Co γ-irradiationand the optimization of its culture medium.Journal of Chemical Engineering of Chinese Universities,2007,21(5):820-825.
[57] 陈咏竹.γ-多聚谷氨酸生产菌的诱变选育及重金属吸附的应用研究.成都:四川大学,2005. Chen Y Z.Mutation breeding of production of γ-poly(glutamic acid) and applied study on absorption of Cu2+ by γ-poly glutamic acid.Chengdu:Sichuan University,2005.
[58] 张姝,李楠,黄登禹,等.γ-聚谷氨酸高产菌株Bacillus natto S003-D(16)的选育和优化.中国酿造,2009,202(1):70-73. Zhang S,Li N,Huang D Y,et al.Screening of high yield strain Bacillus natto S003-D16 producing γ-Polygluamic acid and its optimization of fermentation conditions.China Brewing,2009,202(1):70-73.
[59] 杜沛,宴正,陈双喜.γ-聚谷氨酸高产菌株的选育及发酵条件优化.河南大学学报(自然科学版),2010,40(2):179-184. Du P,Yan Z,Chen S X.High production strain breeding for γ-polyglutamic acid and its fermentation conditions optimization.Journal of Henan University (Natural Science),2010,40(2):179-184.
[60] 李楠,黄登禹,李飞,等.γ-聚谷氨酸产生菌S004-50-01的筛选和优化培养.食品与发酵工业,2006,32(6):1-4. Li N,Huang D Y,Li F,et al.Screening of Bacillus natto S004-50-01 producing γ-polyglutamic acid and optimization of fermentation culture.Food and Fermentation Industries,2006,32(6):1-4.
[61] 疏秀林,施庆珊,冯静,等.一株非谷氨酸依赖型聚γ-谷氨酸高产菌株的鉴定与诱变育种.微生物学通报,2009,36(5):705-710. Shu X L,Shi Q S,Feng J,et al.Identification and simulation mutation of a high-productive strain of poly (γ-glutamic acid) independent of glutamic acid.Microbiology China,2009,36(5):705-710.
[62] 黄金,陈宁. γ-聚谷氨酸的性质与生产方法.发酵科技通讯,2005,26(4):44-48. Huang J,Chen N.The property and production of γ-polyglutamic acid.Fermentation Technology Communication,2005,26(4):44-48.
[63] Sanda F,Fujiyama T,Endo T.Chemical synthesis of poly-gamma-glutamic acid by polycondensation of gamma-glutamic acid dimer:Synthesis and reaction of poly-gamma-glutamic acid methyl ester.Journal of Polymer Science PartA-polymer Chemistry,2001,39(5):732-741.
[64] 张富仓,康绍忠.BP保水剂及其对土壤与作物的效应.农业工程学报,1999,15(2):74-78. Zhang F C,Kang S Z.Water retaining BP agent and its effect on soil and crops.Transaction of the CSAE,1999,15(2):74-78.
[65] 张斌,金莉.固定化酶及其在食品中的应用.中国食品添加剂,2006,4(1):147-150. Zhang B, Jin L.Immobilized enzyme and its application in the food industry.China Food Additives,2006,4(1):147-150.
[66] 刘静,程显好,刘伟,等.固态发酵生产多聚谷氨酸培养条件的优化.食品与药品,2011,13(3):85-89. Liu J,Cheng X H, Liu W,et al.Optimization of solid fermentation conditions to produce poly-γ-glutamic acid.Food and Drug,2011,13(3):85-89.
[67] 王浩,杨丽萍,乔君,等.γ-聚谷氨酸的研究进展.山东食品发酵,2011,163(4):30-34. Wang H,Yang L P, Qiao J,et al.Study of Poly-γ-glutamic acid.Shandong Food Fermentation,2011,163(4):30-34.
[68] Meshnick S K, Smith C.Capacity of a cis-diammineplatinum(II)-poly-glutamic acid complex to cure trypanosome congolense infection in mice.Antimicrobial Agents and Chemotherapy,1984,25(2):286-288.
[69] Avichezer D, Amon R.Functional polymers in drug delivery:carier-support CDDP(cis-platin) complexs of polycarboxslates effect on human ovarian carcinoma.React Funct Polym,1998,36(1):59-69.
[70] 彭银仙,徐虹,陈国广,等.新型药物载体聚谷氨酸的合成及其应用.中国新药杂志,2002(7):515-519. Peng Y X,Xu H,Chen G G,et al.Synthesis and application of new drug carrier polyglutamic acid.Chinese Journal of New Drugs,2002(7):515-519.
[71] 叶海峰.γ-聚谷氨酸-顺铂复合物的制备及其抗肿瘤活性研究.上海:上海华东师范大学,2007. Ye H F.Preparation and anticancer activity of poly(γ-glutamic acid)-cisplatin conjugate.Shanghai:Shanghai East China Normal University,2007.
[72] Yamaguchi S,Tatumi T,Takehara T,et al.Eph A2 derived peptide vaccine with amphiphilic poly (γ-glutamic acid) nanoparticles elicits an anti-tumor effect against mouse liver tumor. Cancer Immunol Immun,2010,59(5):759-767.
[73] Mitsuiki M,Mizuno A,Tanimoto H,et al. Relationship between the antifreeze activities and the chemical structures of oligo and poly(glutamic acids).Journal of Agricultural and Food Chemistry,1998,46(3):891-895.
[74] Yao J,Xu H,Wang J,et al.Removal of Cr(IH), Ni(II) and Cu(II) bypoly (γ-glutamic acid) from Bacillus subtilis NX-2. J Biomat Sci,2007,18(2):193-204.
[75] 张绪瑛,刘雯,黄静.γ-聚谷氨酸锰的制备及其性质研究.云南大学学报,2009,31(2):204-207. Zhang X Y,Liu W,Huang J.Diversity of heteropteran communities in lac plantation-farmland ecosystem.Journal of Yunnan University,2009,31(2):204-207.
[76] 王传海,何都良,郑有飞,等.保水剂新材料γ-聚谷氨酸的吸水性能和生物学效应的初步研究.中国农业气象,2004,25(2):19-22. Wang C H,He D L,Zhang Y F,et al.A preliminary study on water absorption properties and biological effects of a new water-holding agent γ-poly glutamate.Chinese Journal of Agrometeorology,2004,25(2):19-22.
[77] 游庆红,张新民,陈国广,等.γ-聚谷氨酸的生物合成及应用.现代化工,2002,22(12): 56-59. You Q H,Zhang X M,Chen G G,et al.Biosynthesis and application of poly(γ-glutamic acid).Modern Chemical Industry,2002,22(12): 56-59.
[78] 黄金,陈宁.γ-聚谷氨酸的性质与生产方法.氨基酸和生物资源,2004,26(4):44-48. Huang J,Chen N.The Property and production of γ-polyglutamic acid.Amino Acids & Biotic Resources,2004,26(4):44-48.
[79] 汪德生,付蕾,郎威明,等.γ-聚谷氨酸与化学絮凝剂絮凝性能对比研究.安全与环境学报,2007,7(6):48-50. Wang D S,Fu L,Lang W M,et al.Comparative study on flocculation capability between biofiocculanty γ-PGA and chemical.Journal of Safety and Environment,2007,7(6):48-50.
[80] 何观辉.聚谷氨酸与聚谷氨酸水胶-化妆品原料家族中的新"明星".中国化妆品专业版,2006,1(5):14-15. He G H.γ-PGA(gamma-polyglimatic acid) and γ-PGA hydrogel.Chinese Cosmetics Professional Edition,2006,1(5):14-15.
[81] 施庆珊.γ-聚谷氨酸的微生物合成与应用.精细与专用化学品,2004,12(11):20-23. Shi Q S.Biosynthesis and application of C-polyglutamic acid.Fine and Specialty Chemicals,2004,12(11):20-23.

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