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| Detection of Individualized Treatment and Test of Helicobacter pylori Infection through Visualized Gene Chip Technology |
| YAO Xue1, LIU Qi-qi2, ZHAO Qing1, CHEN Su-hong2 |
1. Guangzhou Medical College, Guangzhou 510182, China;
2. Beijing Institute of Radiation Medicine, Beijing 100038, China |
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Abstract Objective: To establish a method of visualized gene chip detection that can simultaneously measure the gene polymorphism of CYP2C19 that is related to clarithromycin and quinolones resistance mutation in Helicobacter pylori infection and to the metabolism of proton pump inhibitor. Method: Through analysis the sequence of human CYP2C19*2, CYP2C19*3 and the targets related to resistance to clarithromycin and quinolone, a gene chip to test the resistence of Helicobacter Pylori was established. The specificity, sensitivity and repeatability of the gene chip was evaluated in the optimized PCR system, with hybridization reaction and visualized detection. Result: The results of detection of 196 clinical samples using gene chips were consistent with that by sequencing. The detection sensitivity of the chip for Hp was 103CFU/ml, and that for human genomic DNA was 2ng/μl.Conclusion: This method was quick, highly specific, sensitive, and economic. With its clinical application potential, it is promising in providing medication guidance for Hp elimination in clinical use and individualized treatment.
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Received: 07 January 2013
Published: 25 April 2013
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