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中国生物工程杂志

China Biotechnology
China Biotechnology  2013, Vol. 33 Issue (3): 111-116    DOI:
    
Assembly of Targeting Vector by Golden Gate Cloning
LIANG Long1, YANG Hua2,3, YANG Yong-lin2,3, LIU Shou-ren2,3, PI Wen-hui 2,3
1. College of Animal Science & Technology,Shihezi University,Shihezi 832003,China;
2. Animal Husbandry and Veterinary Institution, Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China;
3. Key Lab of Sheep Breeding and Reproduction, Xinjiang Agroreclamation Academy of Sciences, Shihezi, Xinjiang 832000, China
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Abstract  

The ‘Golden Gate’ cloning is based on the ability of type IIS restriction enzymes and DNA ligase enzymes to assemble multiple DNA fragments in a defined linear order in one tube and one step. The method is efficient and yields recombinant vectors that do not contain unwanted sequences in the final construct after just a short time restriction-ligation. To edit mouse β casein gene, recombination targeting vectors of the mouse β casein gene targeting locus were constructed by the ‘Golden Gate’ cloning.



Key wordsDNA recombination      ‘Golden Gate’cloning      Mouse &beta      casein gene     
Received: 27 December 2012      Published: 25 March 2013
ZTFLH:  Q78  
Cite this article:

LIANG Long, YANG Hua, YANG Yong-lin, LIU Shou-ren, PI Wen-hui. Assembly of Targeting Vector by Golden Gate Cloning. China Biotechnology, 2013, 33(3): 111-116.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2013/V33/I3/111

[1] Marsischky G, LaBaer J. Many paths to many clones: a comparative look at high-throughput cloning methods. Genome Research, 2004, 14: 2020–2028.
[2] Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with high throughput capability. PLoS ONE, 2008, 3(11): e3647.
[3] Engler C, Gruetzner R, Kandzia R, et al. Golden gate shuffling: a one-pot DNA shuffling method based on type IIS restriction enzymes. PLoS ONE, 2009, 4(5): e5553.
[4] Morbitzer R, Elsaesser J, Hausner J, et al. Assembly of custom TALE-type DNA binding domains by modular cloning. Nucleic Acids Res, 2011, 39(13): 5790-5799.
[5] Szybalski W, Kim S C, Hasan N, et al. Class-IIS restriction enzymes--a review. Gene, 1991, 100: 13-26.
[6] Weber E, Gruetzner R, Werner S, et al. Assembly of designer TAL effectors by golden gate. PLoS One, 2011, 6(5): e19722.
[7] Cermak T, Doyle E L, Christian M, et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res, 2011, 39(12): e82.
[8] Sanjana N E, Cong L, Zhou Y, et al. A transcription activator-like effector toolbox for genome engineering. Nat Protoc, 2012, 7(1): 171-192.
[9] Goff S P, Berg P. Construction of hybrid viruses containing SV40 and lambda phage DNA segments and their propagation in cultured monkey cells. Cell, 1976, 9: 695.
[10] Weber E, Engler C, Gruetzner R, et al. A modular cloning system for standardized assembly of multigene constructs. PLoS One, 2011, 6(2): e16765.

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