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| Optimization Process for Catalytic Esterification of Microbial Lipid |
| LIU Hui-ying, XUE Dong-hua, PAN An-long, XU Hong-zhang, YE Xiao-jin, SUN Guo-ying |
| School of Chemistry and Life Science, Changchun University and Technology, Changchun 130012, China |
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Abstract Microbial lipid fermentation production(cell dry weight of 21.2g/L, microbial lipid content exceed 56.5%) from corn straw hydrolysate of pentose and hexose used efficiently by Mortierella iabellina M2. The process for prepared microbial diesel from microbial lipids by a two-step catalyzed process of acid catalysis deacidification-base catalysis transesterification was focused. The optimal reaction conditions of esterification: the H2SO4 catalyst concentration of 5%, methanol to oil molar ratio 12∶1, reaction temperature is 65℃ and reaction time 2 h, during the optimization of reaction conditions, the acid value from 24.47mgKOH/g drop to 1.6mgKOH/g, then using the Box-Benhnken center unitized design experiment and the response surface methodology(RSM) optimum microbial oil transesterification reaction conditions: the KOH catalyst concentration of 1.25%, methanol to oil molar ratio10∶1, reaction temperature is 57℃, reaction time was 1.47h. The microbial diesel yield higher than 93.39%.
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Received: 05 December 2012
Published: 25 March 2013
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