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| Effects of CAS Silencing by RNAi on the Content of Saponins in Panax notoginseng |
| SUN Ying, GE Feng, LIU Di-qiu, RAO Jian |
| Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China |
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Abstract PNS (panax notoginseng saponins) is the main medical bioactive component from Panax notoginseng. The medical value of PNS cannot be extended because of its low production. From previous studies inferred that both PNS and phytosterol are derived from the precursor 2,3-oxidosqualene. The enzyme cycloartenol synthase (CAS) and dammarenediol synthase (DS) are responsible for PNS and phytosterol biosynthesis, respectively. The CAS RNAi expression vector is established by Gateway technology. By the way of agrobacterium-mediated method, the vector was transferred and integrated into the Panax notoginseng genome to suppress the expression of CAS, block the synthesis of phytosterol, and increase the PNS production indirectly.
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Received: 29 October 2012
Published: 25 March 2013
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