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| The Effect of Down-regulation of GINS2 on Proliferation and Apoptosis of HL60 Cells |
| ZHANG Xi1, LIU Bei-zhong2, GAO Yan-jun1, LI Liang1, GAO Yuan-mei1, HU Xiu-xiu1, MA Peng-peng1, ZHONG Liang 1 |
1. Key Laboratory for Clinical Medical Diagnostics of Ministry of Education,Chongqing Medical University,Chongqing 400016,China;
2. Central Laboratory of Yong-Chuan Hospital, Chongqing Medical University,Chongqing 402160,China |
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Abstract Objective: To investigate the effect and mechanism of small interfering RNA targeted GINS2 expression on proliferation and apoptosis of human acute promyelocytic leukemia HL60 cells. Method: The expression of GINS2 complexes comprised in three leukemia cell lines HL60 ,U937,THP-1 were detected by Reversed-Transcript PCR(RT-PCR).The plasmid specific targeted GINS2 expression were stably transfected into HL60 cells with high expression of this gene by means of lipidosome-mediated method. Then,the transcription and translation level were detected by RT-PCR and Western blot respectively. Meanwhile cell proliferation and apoptosis after transfection were measured by MTT method and flow cytometry. In addition,the change of apoptin Bax and Bcl2 were disclosed by western blot. Results Among three leukemia cell lines ,the expression of GINS2 from low to high was following as:THP-1,U937,HL60. After siRNA plasmid were transfected into HL60 cells,both GINS2 expression level of mRNA and protein in interfering group ,measuring by RT-PCR and Western blot method ,were down-regulated after transfection when compared with untreated control group and negative control group. Together, MTT and flow cytometry showed that cell growth was significantly inhibited.(P＜0.05). Moreover,the expression lever of Bax was significantly increased whereas Bcl2 was dramatically decreased in positive transfected group.Conclusion: The down-regulation of mRNA and protein level of GINS2 complexes might induce apoptosis and inhibit cell growth by ways of up-regulation of Bax and down-regulation of Bcl2 in HL60 cell lines.
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Received: 21 September 2012
Published: 25 March 2013
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