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| The Abilities of ShDNMT1 to Promote Reprogramming are Enhanced by Vitamin C-recused Proliferation |
| LI Ling-yu1,2, HE Song-wei2, ZHENG Hui2 |
1. School of Life Science, Anhui University, Hefei 230601, China;
2. Guangzhou Institute of Biomedicine and Health, CAS, Guangzhou 510530, China |
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Abstract DNA methylation and Vitamin C (Vc) play critical roles during the generation of induced pluripotent stem cells (iPSCs). In current study, the interaction between DNMT1 and Vc during reprogramming process was studied. In the absence of Vc, reducing DNMT1 expression with shRNA did not promote the transition from fibroblasts or pre-iPSC to iPSCs obviously. While when Vc was added in the medium, shDNMT1 increased the generation of iPSCs from both kinds of cells. In addition, shDNMT1 actually inhibited cell proliferation and increased the percentage of cells in G1 phase, which reduced its ability to promote reprogramming partially. Therefore, by promoting cell proliferation, decreasing the percentage of cells in G1 phase, and rescuing the inhibitory effect of shDNMT1 on proliferation, Vc further enhanced the ability of shDNMT1 to promote reprogramming.
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Received: 01 December 2015
Published: 16 March 2016
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