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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (02): 38-42    DOI:
    
Cloning, Expression and Function Analysis of Serpin Gene in Bifidobacterium bifidum
YE Ruo-song, LI Peng, ZHANG Zhao-lin, WAN Cui-xiang, CUI Jia, WEI Hua
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
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Abstract  

The Serpin gene has been cloned from the genomic DNA of Bifidobacterium bifidum WBBI02. A recombinant Serpin expression system in prokaryotic cells, namely, pBX2-WBBI02 was constructed, and the Serpin protein was successfully expressed and purified. An in vitro inhibition test of Serpin against intestinal proteinase and the effect of Serpin on the adherence of Bifidobacterium longum were approached. The results showed that the serpin gene of Bifidobacterium bifidum WBBI02 is 768 bp, whose similarity is 99.9% with that of Bifidobacterium longum NCC2705. The purified Serpin was efficient to inhibit the activities of eukaryotic α-chymotrypsin and pancreatic elastase by maximum 90% and 97%, respectively, microscopical observation proved that Serpin enhanced the adherence of Bifidobacteria to HT-29 cells.



Key wordsBifidobacteria      Serpin      Clone and expression      Adherence     
Received: 14 September 2010      Published: 18 February 2011
ZTFLH:  Q789  
Cite this article:

YE Ruo-song, LI Peng, ZHANG Zhao-lin, WAN Cui-xiang, CUI Jia, WEI Hua. Cloning, Expression and Function Analysis of Serpin Gene in Bifidobacterium bifidum. China Biotechnology, 2011, 31(02): 38-42.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I02/38

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