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| Advances of the Technology of Spermatogonial Stem Cell Transplantation and Recipient Preparation |
| WANG Chen1,2, ZHU Hua-bin1, LIU Ling1, HAO Hai-sheng1, ZHAO Xue-ming1, FEN Rong3, DU Wei-hua1, QIN Tong1, LIU Yan1, LI Zong-qiang2, WANG Dong 1 |
1. The key laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193,China;
2. Animal Science and Technology College, Guangxi University, Nanning 530004,China;
3. Wulanchabu Vocational College, Jining 012000, China |
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Abstract Spermatogonial stem cell (SSCs) transplantation laid the foundation for the study on spermatogenesis, male fertility and the new transgenic technology. Mature technology system of SSCs transplantation has been established in mice. The receptor preparation method of busulfan treatment, and the transplantation methods of seminiferous tubule injection and rete testis injection have been widely used in this scientific area. However, busulfan treatment can lead to a high mortality ; It costs much to prepare receptor animal using local irradiation and the animal emptied from endogenous stem cells; The receptor preparation method of heat shock is limited for application and its effect is unstable. All three transplantation methods require high operating techniques. Inject seminiferous tubule and efferent ducts need apparatus for microinjecting testis, while the catheter should be inserted into the rete testis under ultrasound guidance.The transplantation results are quite distinct among different experiments and species, and the efficiency needs to be further improved. The immunological rejection after transplantation also should be explored. A deep study on testicular structure and biological characteristics of SSCs will help to establish novel, simple and efficient methods for receptor preparation and transplantation of SSCs.
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Received: 19 March 2012
Published: 25 August 2012
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Cite this article:
WANG Chen, ZHU Hua-bin, LIU Ling, HAO Hai-sheng, ZHAO Xue-ming, FEN Rong, DU Wei-hua, QIN Tong, LIU Yan, LI Zong-qiang, WANG Dong. Advances of the Technology of Spermatogonial Stem Cell Transplantation and Recipient Preparation. China Biotechnology, 2012, 32(08): 81-86.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I08/81
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