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| Interaction among BRPF1, Its Novel Transcript BRPF2 and RHOX5 Protein |
| GUO Fen1, LIN Pi-rong2, LI Yue-qin2, SU Xian-li2, WANG Ding-ding1, ZHOU Tian-hong 2 |
1. College of Life Science and Bio-pharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China;
2. National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China |
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Abstract RHOX5 is the founding member of the reproductive homeobox on the X chromosome (RHOX) gene cluster. It is selectively expressed in male and female reproductive tissues. Studies based on its expression model indicated a crucial role of RHOX5 in the development of reproductive tissues and spermatogenesis. However, the molecular mechanisms underlying RHOX5 functions remained unclear. A novel transcript of BRPF1, BRPF2, was discovered interacting with RHOX5 protein during the screening of mouse 7 days embryo cDNA library using RHOX5 protein as bait. pGBKT7-BRPF2 plasmid was constructed and was used to confirm the interaction with RHOX5 using yeast two hybrid assay. In addition, the direct interaction between RHOX5 and BRPF2 was confirmed by GST-pull down assay. Moreover, BRPF1 gene was amplified and used to construct pGBKT7-BRPF1 and pGADT7-BRPF1 recombinant plasmids. The interaction between BRPF1 and RHOX5 was determined by yeast two hybrid assay and GST-pull down assay. The confirmation of the interaction between BRPF1, BRPF2 and RHOX5 suggested that BRPF2 may compete with BRPF1 for the interaction with RHOX5, which opens up the functional study on BRPF1, BRPF2 and RHOX5.
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Received: 09 April 2012
Published: 25 September 2012
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