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| Study on Agrobacterium-mediated Transformation of psy Gene into Shoot Meristem of Maize |
| WANG Ting-ting1,2, JI Jing2, WANG Gang2, GUAN Chun-feng2, ZHANG Lie3, JIN Chao 2 |
1. School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;
2. Research Institute of Genetic Engineering,Tianjin University,Tianjin 300072,China;
3. Tianjin Kemel Jinfeng Seed Industry Co. Ltd,Tianjiin 300384,China |
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Abstract The psy gene was transferred into the shoot meristems of two different maize inbred lines including maternal Tian tawu and 7922 by Agrobacterium-mediated transformation. Factors influencing transformation efficiency were optimized.The results indicated that the optimal transformation condition is infection time with vacuum at 20 min and co-cultivation time for 3 days.The transgenic plants were obtained after selected by 200mg/L PPT and 16 of them were confirmed positive by PCR amplification. RT-PCR and HPLC analysis results showed that psy gene had been integrated into the maize genome and transcribed normally, the total carotenoid content in transgenic maize was increased 25% compared to the wild type. This method eliminates the tedious process of tissue culture and proves to be a simple, strong operable transformation method.
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Received: 13 March 2012
Published: 25 August 2012
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