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| RIOK3 Promotes the Cleavage-activation of Caspase-10 on PAK2 |
| LIN Ying, LI Pu, SHAN Jing-xuan, CHEN Xiao-jing, SHI Hui-li, HUO Ke-ke |
| State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China |
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Abstract RIO3, a member of atypical protein kinase, is only discovered in multicellular eukaryotes. The human liver cDNA library was screened with pDBLeu-RIOK3 as bait plasmid by yeast two-hybrid system and PAK2 was identified as a RIOK3 interactive protein. The interaction was confirmed by co-immunoprecipitation assays and immunofluorescent localization experiments. Results of real-time quantitative PCR and Western blot showed that RIOK3 can reduce the amount of PAK2 at protein expression level. CCK-8 and apoptosis detection experiment showed that co-expression of RIOK3 and PAK2 can inhibit cell proliferation and promote apoptosis,and the apoptotic effect can be inhibited by caspase-10G, which is the minimum isoform of caspase-10 and had no enzyme activity. The experimental results showed that RIOK3 may promote cleavage of caspase-10 on PAK2 and play an important role in the PAK2 cleavage-activation pathway.
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Received: 09 April 2012
Published: 25 August 2012
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