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| Progress on Prion Synthesis in vitro |
| LIU Dong, LU Shi-ying, ZHOU Yu, GONG Bin-bin, REN Hong-lin, LIU Zeng-shan |
| Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China |
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Abstract Prion diseases are neurodegenerative diseases, harmful to animal and human health. Mechanisms of prion diseases are not fully understood. Systems of prion replications in vitro are used to studying prion pathogenesis, however, mock environment in vitro for studying conversion mechanisms from normal cellular prion proteins to disease-associated scrapie prion proteins is relatively difficult and very important. Cell-free conversion assay, cell-lysate conversion assay, Protein Misfolding Cyclic Amplification, Autocatalytic conversion assay, several methods of prion conversion in vitro were described, and discussed them in reflecting prion propagation in vivo, a lot of target samples for studying Prion disease were provided, in order to facilitate to further study prion pathogenesis in the future.
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Received: 18 June 2010
Published: 25 October 2010
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Corresponding Authors:
REN Hong-lin, LIU Zeng-shan
E-mail: zsliu1959@163.com;renhl@yahoo.cn
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