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| Study on Peste Des Petits Ruminants Virus Nucleic Acid Detection Based on Gold-nanoparticle Conjuncted Probe |
| TAO Chun-ai, QIU Wen-ying, LI Gang, TIAN Kang-le |
| State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences/ Beijing Scientific Observation and Experiment Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing 100193, China |
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Abstract In order to investigate a rapid method of detecting peste des petits ruminants virus(PPRV) with gold-nanoparticle conjuncted probe,two sets of specific oligonucleotide probes complementary to the ends of the highly conserved region of the PPRV nucleoprotein gene were designed. One was biotin-modified,the other was thioled and conjuncted to gold nanoparticles.During the course of hybridization, the two labeled probes binded to the target nucleic acid respectively, forming a "sandwich"type complex, which was amplified by silver staining after incubated to streptavidin coated immuno-Microwells. Concentration of the target nucleic acid was determined by visual light microscope and spectrophotometry observation. The established method took around 1.5 h and the detection limit for nucleic acid was 10 fmol/L. It would provide theoretical and technical support for the clinical detection of PPRV since it is easy to operate and highly sensitive.
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Received: 21 December 2011
Published: 25 July 2012
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