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| Transformation and Genetic Analysis of Maize with the Lycb |
| HUO Pei, JI Jing, WANG Gang, GUAN Chun-feng, JIN Chao |
| School of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, China |
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Abstract Lycb gene was transformed into embryonic callus derived from Tianta Fifth inbred lines by Agrobacterium-mediated transformation, and the independent T0 transformants and their progenies genetics were analyzed. PCR analysis with 27 T0 transformed plants testified 8 positive plantlets. T1 plantlets were selected by 200 mg/L PPT and seeds of the resistance plants were harvested. T2 transgenic plants were analyzed using PCR, RT-PCR, and the results showed that the six positive lines detected were further confirmed to have PPT resistance. HPLC analysis showed the total beta carotene content from transformed leaves was significantly higher than that of the wild type. The results show that the Lycb gene was able to inherit in the T2 progeny, and transcribed and translated effectively.
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Received: 12 March 2012
Published: 25 July 2012
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