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| The Study of Therapy Efficacy Applied Sheep Amnion Epithelial Cells in Rabbit Radius Defected Models |
| ZHU Xue-min, LIU Jun-ping, YANG Yin-feng, WANG Xiu-mei, MI Yan, LI Hai-jun, LI Yan, MENG Qing-gang, CAO Gui-fang |
| Laboratory of Animal Embryo and Developmental Biology, Inner Mongolia Agricultural University, Huhhot 010018, China |
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Abstract To explore the potentials differentiation of sheep amnion epithelial cells into bone in vitro, the sheep amnion epithelial cells of stem cells properties were injected into New Zealand rabbit radius defected models and then observed the radius restoration. The sheep amnion epithelial cells were cultured and made morphological identifications. The rabbit’s left 13-centimetre long radius defected models were artificially divided into high dose group, low dose group and control group randomly. Sheep amnion epithelial cells with the dense of 5×107 and 5×106 in 5ml saline and 5ml saline were injected into high dose group of rabbits, low dose group of rabbits and control group of rabbits respectively. Took the models for X-ray in 2 weeks, 4 weeks and 8 weeks after the sheep amnion epithelial cells transplantations respectively and observed the radius restorations. At the mean time, the radius defected areas were observed by histological methods, and the trabecular bones generating quantities and the bone remodeling time were analysised. Defected radiuses in high dose group of models were restored completely at 8 weeks after the sheep amnion epithelial cells transplantations. Meanwhile, the quality and quantities of trabecular bones in high dose group were better and higher than in the low dose group and it was the same situation between the low dose group and the control group. Thus it can be seen that the sheep amnion epithelial cells can not only be transplanted among various species of animal, but also are effective for defective bone restoration.
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Received: 09 March 2012
Published: 25 July 2012
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Cite this article:
ZHU Xue-min, LIU Jun-ping, YANG Yin-feng, WANG Xiu-mei, MI Yan, LI Hai-jun, LI Yan, MENG Qing-gang, CAO Gui-fang. The Study of Therapy Efficacy Applied Sheep Amnion Epithelial Cells in Rabbit Radius Defected Models. China Biotechnology, 2012, 32(07): 31-36.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I07/31
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