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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2012, Vol. 32 Issue (03): 76-82    DOI:
    
The Medium Optimization of Cellulases Fermentation of Neurospora crassa by Response Surface Methodology
GU Rui-meng1,2, LI Yong-hao2,3, TIAN Chao-guang2
1. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;
3. Bioengineering and Agricultural Engineering College, Jilin University, Changchun 130022, China
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Abstract  

Neurospora crassa is the native degrader of cellulose, it has capability to produce the mainly components of cellulases, such as CBHs, endoglucanses and β-glucosidases. It has some studies focus on the mechanism or cellulases productivity in Neurospora crassa, but the cellulases fermentation by this fungus is far from optimized. Here, The Response Surface Methodology(RSM) to optimize the medium for cellulased production in FGSC.2489. Combined the Plackett-Burman and Central Composite Design design, the peptone, yeast extract have significant effects on enzyme activity were found. The optimum amount of the Peptone is 7.27g/L and Yeast extract is 5.51g/L in medium. Based on the optimized medium, the cellulases production have significantly improved near 2 folds, from 0.64FPU to 1.27 FPU.

Key words： Neurospora crassa      Response surface methodology      Cellulases     
Received: 03 November 2011      Published: 25 March 2012
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Cite this article:

GU Rui-meng, LI Yong-hao, TIAN Chao-guang. The Medium Optimization of Cellulases Fermentation of Neurospora crassa by Response Surface Methodology. China Biotechnology, 2012, 32(03): 76-82.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2012/V32/I03/76


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