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| Establishment of Bone Marrow Mesenchymal Stem Cells Expressing Neurotrophic Factor Neurturin and Activity Detection in Vitro |
| WANG Wan-pu, SUN Mao-sheng, LI Hong-jun, XIE Tian-hong, YAN Min, ZHOU Yan, YIN Na |
| Laboratory of Biomolecular, Peking Union Medical College, Chinese Academy of Medical Sciences, Institute of Medical Biology, Kunming 650118, China |
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Abstract Study the protection and repairation of damaged neurons which leads to neurodegenerative diseases, neurotrophic factor Neurturin gene was transfected into Rhesus bone marrow mesenchymal stem cells (rMSC) by recombinant adenovirus. The NTN gene could be transcribed and expressed in cytoplasm of rMSC and be secreted which was detected by RT-PCR、immunofluorescence、Western blotting, and then, the culture of Chicken embryonic dorsal root ganglion test and the culture of Fetal rats midbrain dopaminergic neurons test had indicated that the NTN protein had good biological activity in vitro. The results gave a new method for stem cell transplantation to treat the neurodegenerative diseases caused by neurons damage.
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Received: 09 October 2011
Published: 25 March 2012
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[1] Kotzbauer P T, Lampe P A, Heukeroth R O, et al. Neurturin, a relative of glial cell line derived neurotrophic factor. Nature, 1996, 384(6608):467-470.
[2] Kordower J H, Herzog C D, Biplob D, et al. Delivery of Neurturin by AAV2 (CERE-120)-Mediated Gene Transfer Provides Structural and Functional Neuroprotection and Neurorestoration in MPTP-Treated Monkeys. Ann Neurol, 2006,60(6):706-715.
[3] Pereira R, Halford K, O’ Hara M, et al. Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone,cartilage, and lung in irradiated mice. Proc Natl Acad Sci USA,1995,92 (11):4857-4861.
[4] Prockop D. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science, 1997,276 (5309):71-74.
[5] Pittenger M, Mackay A, Beck S C, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999,284 (5411):143- 147.
[6] Richards M, Huibergtse B, Caplan A, et al. Marrow-derives progenitor cell injections enchance new bone formation during distraction. J Orthop Res,1999,17 (6):900-908.
[7] Li Y, Chen J, Wang L, et al. Intracerebral transplantation of bone marrow stromal cells in a 1-methyl-4-phenyl-1,2,3,6-tetrahdyropyridine mouse model of Parkinson’s disease. Neurosci Lett, 2001, 316 (2):67-70.
[8] Beal M. Experimental models of Parkinson’s disease. Nat Rev Neurosci, 2001,2(5):325-334.
[9] Liu S, Yu Y, An H, et al. Cloning and identification of a novel ubiquitin-like protein, BMSC-UbP from human bone marrow stromal cells. Immunol Lett, 2003, 86(2):169-175.
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