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| Four Types of Promoter Controlled Campylobacter jejuni Single Hemoglobin Improved the Growth of Escherichia coli |
| XU Li, XIONG Wei, YANG Jiang-Ke |
| College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China |
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Abstract Bacteria hemoglobin could improve the cell's oxygen utility and enhance the cell growth rate under low oxygen environment. To improve the biomass of E. coli and supply a novel type of host cell for bioengineering, the effect of four types of promoter controlled Campylobacter jejuni hemoglobin on the growth of E. coli cells was comparatively analyzed. The C. jejuni hemoglobin chb was initially synthesized and then it was expressed under the control of inducible promoter PT7 and Pvgh, constitutive promoter P2 and PspoI-II. The growth curves of these four recombinant E. coli cell in flask and capped bottle were depicted and founded that the chb gene has been actively expressed in these cells and could significantly improve the growth of cells, which was further testified by the experiment conducted in bioreactor. The relationship between cell density, cell fresh weight and CO differential spectrum value was comparatively analyzed, and the characteristics of these four type of promoter controlled recombinants on the hemoglobin inducible expression and growth regulation were discussed, and a selection on these host cells under different cultivation conditions such as microaerobic and aerobic environments was proposed.
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Received: 09 September 2015
Published: 19 November 2015
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