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| Mutation Screening of Production Strains of Spinosad Based on Ribosome Engineering Theory through ARTP |
| QIAO Chang-sheng1,2, ZHAO Nan1, SHI Man-man1, ZHU Ming1, LI Xue2 |
1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China;
2. Tianjin Peiyang Biotrans Co., Ltd., Tianjin 300457, China |
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Abstract Based on the ribosome engineering theory, the atmospheric and room temperature plasma (ARTP) was used in screening of Saccharopolyspora spinosa. Taking the strain Saccharopolyspora spinosa QYLZ 88912 as the original strain, choose the spore suspension of three different irradiation time after ARTP mutation, then mix them to increase the resistance screening probability. A high-yield strain S.spinosa Sg200Rif110St40Er90-028 was obtained after screening to the mixed spore suspension. This strain had the resistance of sulfaguanidine, rifampin, streptomycin, erythromycin. Its production of spinosad reached 1516.93 mg/L under the level of shake flask fermentation through 7 days, which was 148.37% higher than that of the original strain S.spinosa QYLZ 88912. The subculture experiments indicated that the hereditary characteristic of high productivity of S.spinosa Sg200Rif110St40Er90-028 was relatively stable. The fermentation materials of spinosad producing strain were optimized by the response surface methodology based on the single factor experiment. Under the optimized medium, the spinosad was increased by 286.71% compared with the previous condition.
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Received: 24 October 2013
Published: 25 January 2014
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