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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (2): 38-42    DOI: 10.13523/j.cb.20160206
    
Ectopical Expression of Silkworm Male-specific dsxM3 and Its Effect on Downstream Targets
DUAN Jian-ping1,2, LI Ying2, KAN Yun-chao2, YAO Lun-guang2, XIA Qing-you1
1. State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China;
2. Henan Provincial Key Laboratory of Funiu Mountain Insect Biology, Nanyang Normal University, Nanyang 473061, China
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Abstract  

Doublesex gene dsx controls the somatic sexual development by sex-specifically alternative splicing. The expression of Bmdsx was analysed and a novel male-specific splice form BmdsxM3 was also found, but whether it has biological function is unknown. Here, two transgenic lines T1 and T2 were constructed to ectopically express BmdsxM3 in females. As a result, sex reversal and development abnormality all failed to turn up, but the expression levels of SP1 and Vg were downregulated in the fat body of transgenic females, and the expression level of PBP was upregulated in the antenna of transgenic females. All the results indicated that the novel splice form BmdsxM3 had the role of regulation of downstream target gene expression, and it took part in the somatic sexual development in silkworm, Bombyx mori.



Key wordsBombyx mori      Target gene      doublesex gene      Transgene     
Received: 01 September 2015      Published: 19 November 2015
ZTFLH:  Q789  
Cite this article:

DUAN Jian-ping, LI Ying, KAN Yun-chao, YAO Lun-guang, XIA Qing-you. Ectopical Expression of Silkworm Male-specific dsxM3 and Its Effect on Downstream Targets. China Biotechnology, 2016, 36(2): 38-42.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160206     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I2/38

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