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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (10): 100-105    DOI:
    
Establishment of Event Specific Qualitative PCR for Detecting Genetically Modified Wheat B73-6-1
HUO Nan, ZHANG Ming-hui, LIU Ying, QIU You-wen, AO Jin-xia, QU Bo, GAO Xue-jun
Northeast Agricultural University, Life Science and Biotechnique Research Center, Testing and Inspection for GMO of Ministry of Agriculture of P.R.C., Haerbin 150036, China
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Abstract  

By Chromosome walking PCR technique, the 3'flanking sequence of the exogenous gene in the pAHC25 plasmid of wheat ‘B73-6-1’ was successfully cloned. An event specific transgenic detection method for ‘B73-6-1’ was established by PCR with the specific primers based on the 3′border. The amplification fragment include part sequence of both the exogenous DNA and wheat genome. This assay was applied to detect typical genetically modified crops, with high specificity for B73-6-1, and the limit of detection was 0.1%. The results showed that the qualitative PCR assay was accurate, rapid and efficient for detection of B73-6-1.



Key wordsGenetically modified wheat      B73-6-1      Chromosome walking      Event-specific      PCR detection     
Received: 31 May 2011      Published: 25 October 2011
ZTFLH:  Q819  
Cite this article:

HUO Nan, ZHANG Ming-hui, LIU Ying, QIU You-wen, AO Jin-xia, QU Bo, GAO Xue-jun. Establishment of Event Specific Qualitative PCR for Detecting Genetically Modified Wheat B73-6-1. China Biotechnology, 2011, 31(10): 100-105.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I10/100


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