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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (10): 95-99    DOI:
    
Establishment of Protein Fingerprint Database of Common Clinical Gram Positive Cocci
YAN Hui1, HUANG Wen-fang2, YANG Yong-chang2, XIAO Dai-wen2, JIANG Wei2, LUO Chun-li1
1. Laboratory Medicine Department, Chongqing Medical University, Chongqing 400016, China;
2. Clinical Laboratory Department, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China
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Abstract  

To establish a protein fingerprint database of common clinical Gram positive cocci, which can be applied for rapid identification of bacteria, 185 clinical bacterial isolates including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Enterococcus faecium, Enterococcus faecalis were collected and studied. These isolates were assigned into training set and testing set. Bacterial proteins were detected by surface enhanced laser desorption & ionization time-of-flight mass spectrometry (SELDI-TOF MS). Protein fingerprint was analyzed by ProteinChip and Biomarker Wizard software and a protein fingerprint database of some Gram positive cocci was established by using self-constructed Fingerwave software. Similarity analysis between the protein fingerprint of testing set and the constructed database was conducted to evaluate its diagnostic value. A small protein fingerprint database was developed for five common Gram positive cocci. All the tested strains using the database were successfully identified with a result perfectly consistent with those obtained by using automated microbiology analysis system and molecular biological method. Thus, construction of a large-scale protein fingerprint database is desired, which will provide a practicable tool for rapid identification of bacteria.



Key wordsGram positive cocci      SELDI-TOF MS      Protein fingerprint database     
Received: 23 May 2011      Published: 25 October 2011
ZTFLH:  Q819  
Cite this article:

YAN Hui, HUANG Wen-fang, YANG Yong-chang, XIAO Dai-wen, JIANG Wei, LUO Chun-li. Establishment of Protein Fingerprint Database of Common Clinical Gram Positive Cocci. China Biotechnology, 2011, 31(10): 95-99.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I10/95


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