|
|
Effects of Nitrogen-containing Media on the Accumulation of Lipid and Carbohydrate in Marine Microalgae Isochrysis zhanjiangensis |
FENG Di-na1,2, AI Jiang-ning1, LIU Ya-nan1, CHEN Zhao-an1, XUE Song1, ZHANG Wei3,4 |
1. Marine Bioproducts Engineering Group, Dalian Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China;
3. Flinders Centre for Marine Bioprocessing and Bioproducts (FCMB2) Flinders University, Aldelaide SA5042, Australia;
4. Department of Medical Biotechnology, School of Medicine, Flinders University, Adelaide SA5042, Australia |
|
|
Abstract Effects of eight media on the growth, PSⅡ activity, lipids and carbohydrate accumulation of a marine microalgae Isochrysis zhanjiangensis were investigated. When the nitrogen concentration in the medium is 1.5 g/L, the highest lipid content of 39.8 % and the maximum lipid yield of 0.92 g/L were achieved, while the carbohydrate content of 11.6 % was the lowest. When the nitrogen concentration in the medium is 0.016 g/L, the lowest biomass of 0.74 g/L and the lowest lipid content of 21.1 % were obtained. However, the highest carbohydrate content of 44.4 % was obtained. Based on the result of linear fitting equation, the concentration of NO3-in the medium has a good positive correlation with total lipid content of algal cells. Therefore, the regulation in the yield of lipids and carbohydrate is realized by studying different nitrogen-level media.
|
Received: 08 July 2011
Published: 25 October 2011
|
|
|
|
[1] Chisti Y. Biodiesel from microalgae. Biotechnol Adv, 2007, 25: 294-306.
[2] Teresa M, Anto'nio A M, Nidia S C. Microalgae for biodiesel production and other applications: A review. Renew Sust Energ Rev, 2010, 14: 217-232.
[3] Hu Q, Sommerfeld M, Jarvis E. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J, 2008, 54: 621-639.
[4] Li Y Q, Horsman M, Wang B. Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biotechnol, 2008, 81:629-636.
[5] Solovchenko A E, Goldberg I K, Cohen S D. Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalgae Parietochloris incise. J Appl Phycol, 2008, 20:245-251.
[6] Lv J M, Cheng L H, Xu X H, et al. Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions. Bioresour Technol, 2010, 101: 6797-6804.
[7] Fidalgo J P, Cid A, Torres E, et al. Effects of nitrogen source and growth phase on proximate biochemical composition, lipid classes nd fatty acid profile of the marine microalga Isochrysis galbana. Aquaculture, 1998, 166: 105-116.
[8] 杨泽民,章群,谢数涛.湛江等鞭金藻18SrRNA基因序列分析与分类学意义.水利渔业,2008,28(2):13-15. Yang J M, Zhang Q, Xie S T. Reservoir Fisheries, 2008,28(2):13-15.
[9] 李文权,王宪,陈清花,等. 超声波对湛江等鞭金藻生长和脂肪酸组成的影响.海洋学报, 2002,24(3):94-100. Li W Q,Wang X,Chen Q H, et al. Acta Oceanologica Sinica, 2002, 24(3):94-100.
[10] Zheng J, Hao J M, Wang B, et al. Bioremediation of aquiculture wastewater by microalgae Isochrysis zhanjiangensis and production of the biomass material.Key Engineering Materials,2011, 460: 491-495.
[11] Takagi M, Watanabe K, Yamaberi K, et al. Limited feeding of potassium nitrate for intracellular lipid and triglyceride accumulation of Nannochloris sp. UTEX LB1999. Appl Microbiol Biotechnol, 2000, 54: 112-117.
[12] 刘远, 陈兆安,陆洪斌,等.亚心形扁藻培养基的优化及光合特性。过程工程学报,2007,7(6):1197-1201. Liu Y, Chen Z A, Lu H B, et al. Chinese Journal of Process Engineering, 2007,(6):1197-1201.
[13] Sheehan J, Dunahay T, Benemann J, et al. A look back at the U.S. department of energy's aquatic species rogram-Biodiesel from algae. National Renewable Energy Laboratory, 1998.
[14] Zhu M, Zhou P P, Yu L J. Extraction of lipids from Mortierella alpina and enrichment of arachidonic acid from the fungal lipids. Bioresour Technol, 2002, 84:93-95.
[15] 尹翠玲,梁英,张秋丰.氮浓度对球等鞭金藻3011和8701叶绿素荧光特性及生长的影响.水产科学,2008,27(1):28-31. Yin C L, Liang Y, Zhang Q F. Fisheries Science, 2008,27(1):28-31.
[16] 郑阳,陈兆安,傅赘彬,等.亚心形四爿藻培养和产氢过程一体化平板光生物反应系统.高等学校化学学报,2008,29(11):2209-2212. Zheng Y, Chen Z A, Fu Y B, et al. Chemical Research in Chinese University, 2008, 29(11):2209-2212.
[17] Beardall J, Berman T, Heraud P, et al. A comparison of methods for detection of phosphate limitation in microalgae. Aquat Sci, 2001, 63: 107-121.
[18] Lin Y H, Chang F L, Tsao C Y, et al. Influence of growth phase and nutrient source on fatty acid composition of Isochrysis galbana CCMP 1324 in a batch photoreactor. Biochem Eng J, 2007, 37: 166-176.
[19] Rodolfi L, Chini G, Niccolo Bassi Z. Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng, 2009, 102:101-112.
[20] Li Y T, Han D X, Sommerfeld M, et al. Photosynthetic carbon partitioning and lipid production in the oleaginous microalga Pseudochlorococcum sp. (Chlorophyceae) under nitrogen-limited conditions. Bioresour Technol, 2011, 102: 123-129.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|