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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (9): 8-13    DOI:
    
Overexpression of Heat Shock Protein70 Enhances Production of Antibody in Chinese Hamster Ovary Cells
HUANG Fen1,2, AN Xiao-ping1, LI Chun1, HE Zhang-hua2, ZHANG Bao-zhong1, MI Zhi-qiang1, WANG Juan1,2, TONG Yi-gang1
1. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China;
2. College of Life Science, Shaanxi Normal University, Xi’an 710062, China
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Abstract  

The production of recombinant antibody was significantly enhanced by the method of overexpression of Heat Shock Protein 70 in Chinese Hamster Ovary (CHO) cells. The gene HSP70 was cloned from CHO/dhfr-cells, then HSP70 expression plasmid pcDNA3.1-HSP70 was constructed and transfected into CHO/dhfr-cells. CHO cell line overexpressing HSP70 stably was isolated and the expression of HSP70 gene was analyzed through RT-PCR. Anti-HBs chimeric antibody expression plasmids were transfected into the HSP70 cell line, ELISA was performed to test the production of anti-HBs antibody. It demonstrated that the overexpression of HSP70 can enhance the production of recombinant antibody in CHO/dhfr-cells (P<0.05).



Key wordsChinese hamster ovary cells      Heat Shock Protein 70      Gene expression Antibody     
Received: 30 May 2011      Published: 25 September 2011
ZTFLH:  Q78  
Cite this article:

HUANG Fen, AN Xiao-ping, LI Chun, HE Zhang-hua, ZHANG Bao-zhong, MI Zhi-qiang, WANG Juan, TONG Yi-gang. Overexpression of Heat Shock Protein70 Enhances Production of Antibody in Chinese Hamster Ovary Cells. China Biotechnology, 2011, 31(9): 8-13.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I9/8


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