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| The Third Generation Sequencing Technology and Its Application |
| ZHANG De-fang1, MA Qiu-yue2, YIN Tong-ming1, XIA Tao1,2 |
1. Key Lab of Forest Genetics and Biotechnology Nanjing Forestry University, Nanjing 210037, China;
2. Forestry Resources and Environment institute, Nanjing Forestry University, Nanjing 210037, China |
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Abstract With the development and application of next generation sequencing technology (NGS), some disadvantages are emerging. The third generation single molecular sequencing technology, to a certain extent, may offset these disadvantages of the next generation sequencing in application. This study elaborated the 4 sequencing principles of the third generation single molecular sequencing technologies, compared the advantages and disadvantages with these sequencing technologies, and presented their application in genome sequencing, methylation research, RNA sequencing and medical research.
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Received: 15 January 2013
Published: 25 May 2013
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