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| Influence of PEG to ELP[I]40 Inverse Temperature Transition |
| LIN Heng1,2, LI Jun-ming2, GE Gao-shun2, ZHANG Li-chao2, SUN Li-hui2, HU Xue-jun2 |
1. College of Life Science and Technology, Dalian University, Dalian 116622, China;
2. Medical College, Dalian University, Dalian 116622, China |
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Abstract Objective: To study PEG on the influence of ELP[I]40 inverse temperature transition (Tt). Methods: The ELP[I]40 gene (a (VPGIG)40 five peptide unit composition) was designed and synthesised, and it was expressed and purified, then the Tt was detected under the condition of different concentrations of PEG. Results: In the ELP 40 final concentration to 25 μmol/L, when PEG concentration was 5%, 10%, 15%, 20%, 25%, theTt was respectively reduced down to 26.5℃ and 22℃, 15.2℃, 8.8℃, 2.5℃ from 29℃. Conclusion: The PEG can enhance the ELP purification efficiency via reducing the Tt.
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Received: 04 January 2013
Published: 25 May 2013
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