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| Preparation and Characterization of Monoclonal Antibody of Listeria monocytogenes |
| DONG Xiang-mei1, SUN Ji-chang2, LIU Chun-mei3, ZHONG Zi-qing1, LAI Wei-hua1, WEI Hua1, XIONG Yong-hua1 |
1. State Key Lab of Food Science and Technology, Nanchang University, Nanchang 330047, China;
2. Jiangxi Province Center for Disease Control and Prevention, Nanchang 330029, China;
3. Wuxi Zodolabs Biotechnology CO., LTD, Wuxi 214000, China |
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Abstract Two strains of mAbs against L. monocytogenes, namely 4A7 and 4H11, are generated by immuned the BALB/c mice using the cell fragments of L. monocytogenes as the immunogen. The titers of mAbs are 1:160 000, 1:20 000 and the subclasses of mAbs are IgG1, IgG2a, respectively. The specificity of the mAbs is evaluated using Dot-ELISA method and the two strains of mAbs show a high infinity to Listeria monocytogenes, Listeria ivanovii, Listeria welshimeri, Listeria seeligeri and Listeria innocua whereas exhibit no cross-reactivity with Listeria grayi and other 30 common bacterias. Western-blot analysis showes the mAbs 4A7 and 4H11can recognize the membrane proteins of L. monocytogenes with the molecular weight of 62 kDa and 32 kDa, respectively. Immuno-gold based transmission electron microscopy further demonstrates that the mAbs are capable of binding the membrane antigens of L. monocytogenes.
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Received: 28 December 2012
Published: 25 May 2013
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