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| Expression of XRN1 Protein and Optimization of Fermentation Medium with Response Surface Method |
| WU Ying-chun, MA Qin-qin, DING Xian-feng, ZHANG Kai, LIU Li-li, GUO Jiang-feng |
| College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China |
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Abstract Response surface method (RSM) was used to optimize the fermentation medium of XRN1 protein expression. The Plackett-Burman design was used to evaluate the effects of eight factors, including yeast extract, calcium sulfate, MgSO4?7H2O, glycerol, potassium sulfate, phosphoric acid, ammonium sulfate and potassium hydroxide. The statistical analysis revealed that the key factors influencing XRN1 protein expression were yeast extract, magnesium sulfate and potassium sulfate. The path of steepest ascent was used to define optimal response region for these three factors, then the optimal level combinations of factors were obtained through Box-Behnken design, the optimal conditions were as follows: yeast extract 0.45%, magnesium sulfate 0.38%, and potassium sulfate 1.4%. Under optimal conditions, biomass of yeast could increase by 0.5 fold and expression of XRN1 protein could increase by 40%.
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Received: 12 October 2012
Published: 25 April 2013
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