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| Screening, Identification and Preliminary Fermentation of a High Succinic Acid Producing Strain |
| SHEN Nai-kun1,2,3, QIN Yan1,2,3, WANG Cheng-hua1,2,3, ZHU Jing1,2,3, LIAO Si-ming1,2,3, HUANG Ri-bo 1,2,3 |
1. State Key Laboratory of Non-Food Biomass and Enzyme Technology, Nanning 530007, China;
2. National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, Nanning 530007, China;
3. Guangxi Key Laboratory of Biorefinery, Nanning 530007, China |
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Abstract A strain producing high succinic acid and less byproduction was isolated by a media containing disodium fumarate as the only carbon source and the high concentration sodium succinate from bovin rumen. Morphology analysis, physiological and biochemical properties and phylogenetic analysis based on the 16S rDNA sequence indicated that the strain belonged to the genus Actinobacillus succinogenes within the family Pasteurellaceae. The sequence similarities was 98.98% with the Actinobacillus succinogenes S.JST. And so it was named as Actinobacillus succinogenes GXAS137. The fermentation conditions were optimized by Orthogonal experimental design. The results showed the strain could produce 38.96 g/L succinic acid from 55 g/L glucose. The strain obtained deserves being extended to application of producing succinic acid.
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Received: 28 May 2012
Published: 25 October 2012
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