Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (4): 60-65    DOI: 10.13523/j.cb.20150409
    
Production of the Isoamyl Alcohol in E.coli by Expression of kivD Gene
XIAO Shi-yuan1,2, XU Jing-liang1, CHEN Xiao-yan1, YANG liu1, YUAN Zhen-hong1
Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China
Download: HTML   PDF(621KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Keto acid decarboxylase is the key enzyme for biological synthesis of isoamyl alcohol, rarely existed in E. coli. Keto acid decarboxylase gene kivD(rbs) obtained from Lactococcuslactis subsp.Lactis genome DNA through reaction of PCR, was inserted into E. coli high expression vector pET-28a(+). The formed pET-kivD(rbs), was then transformed into E. coli BL21(DE3) by hot blow. The activity of keto acid decarboxylase and isoamyl alcohol were detected in the fermentation broth.



Key wordskivD gene      Recombinant plasmid      Transform      Isoamyl alcohol     
Received: 14 January 2015      Published: 25 April 2015
ZTFLH:  Q789  
Cite this article:

XIAO Shi-yuan, XU Jing-liang, CHEN Xiao-yan, YANG liu, YUAN Zhen-hong. Production of the Isoamyl Alcohol in E.coli by Expression of kivD Gene. China Biotechnology, 2015, 35(4): 60-65.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150409     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I4/60


[1] Ingram L O, Aldrich H C, Borges A C C, et al. Enteric bacterial catalysts for fuel ethanol production.Biotechnology Progress, 1999, 15(5): 855-866.

[2] 蔡海龙,王秀清.美国燃料乙醇产量快速增长的原因及对国际玉米市场的影响.中国农业大学学报,2013,30(4):127-132 Cai H L, Wang X Q.The reasons of U.S. fuel ethanol production increase and its impact on international maize market. Journal of China Agricultural University, 2013,30(4):127-132.

[3] Savage N.Fuel options:the ideal biofuel.Nature,2011,474(7352):9-11.

[4] Atsumi S,Hanai T,Liao J C.Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature,2008,451(7174):86-89.

[5] 潘超强,高强,郑春阳,等.产异丁醇关键基因在大肠杆菌中表达的研究.微生物学报,2012,39(7):912-920. Pan C Q, Gao Q, Zheng C Y,et al.Coexpression of two essential isobutanol synthesis genes in Escherichia coli. Microbiology China, 2012,39(7):912-920.

[6] 郑丽娟,陈少云,徐刚,等.利用双启动子载体构建产异丁醇大肠杆菌.中国生物工程杂志,2013,33(8):66 -72 Zheng L J, Chen S H, Xu G, et al. Engineering E.coli for isobutanol production by two-promoter vectors. China Biotechnology, 2013,33(8):66-72.

[7] Nicole N E,Shuchi S H,Anna A E,et al.Metabolic engineering for higher alcohol production.Metabolic Engineering,2014,25:174-183.

[8] Martadela P, Pilar F P, Carmen P.Biochemical and molecular characterization of a-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis. FEMS Microbiology Letters,2004,238(2):367-374

[9] Sorensen H P, Mortensen K K. Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microbial Cell Factories, 2005,4(1): 1-8.

[10] Williams R E, Bruce N C. 'New uses for an old enzyme'——the old yellow enzyme family of flavoenzymes. Annals of Microbiology, 2002,148(6): 1607-1614.

[11] Shine J,Dalgarno L.The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA:complementarity to nonsense triplets and ribosome binding sites.National Academy of Sciences,1974,71(4): 1342-1346.

[12] Mary V M,Maurille J F,David A T.Depletion of Free 30S ribosomal subunits in Escherichia coli by expression of RNA containing shine-dalgarno-like sequences.Jouranl of Bacteriology,2002,184(2):494-502.

[13] 李育阳.基因表达技术.北京:科学出版社,2001.15-16. Li Y Y. Gene Expression Technology. Beijing: Science Press, 2001.15-16.

[14] 吴乃虎.基因工程原理(下).北京:科学出版社,1989.113. Wu N H. Genetic Engineering Principles. Beijing: Science Press,1989.113.

[15] König S. Subunit structure, function and organisation of pyruvate decarboxylases from various organisms. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology,1998, 1385(2):271-286.

[16] Ezeji T, Qureshi N, Blaschek H P.Production of acetone-butanol-ethanol (ABE) in a continuous flow bioreactor using degermed corn and Clostridium beijerinckii.Process Biochemistry,2007, 42(1):34-39.

[17] 林丽华, 郭媛, 庞浩,等.大肠杆菌中表达关键基因产异丁醇的研究. 生物技术,2011, 21(3):19-23. Lin L H, Guo Y, Pang H, et al. The construction of recombinant E.coli producing isobutanol. Biotechnology Bulletin, 2011, 21(3):19-23.

[18] Kevin M S,Kwang M C,James C L.Engineering Corynebacterium glutamicum for isobutanol production. Appl Microbiol Biotechnol,2010, 87(3):1045-1055.

[19] 袁振宏,许敬亮,陈小燕,等.一种α-酮酸脱羧酶KIVD-LL及其编码基因和应用,中国, 中华人民共和国国家产权局, CN201410309574,2014,7. Yuan Z H, Xu J L, Chen X Y, et al. An alpha keto acid decarboxylase KIVD-LL and its coding gene,application. China, The national property office of the People's Republic of China, CN201410309574,2014,7.

[20] Liao J C, Connor M R. Engineering of an Escherichia coli strain for the production of 3-methyl-1-butanol. Applied and Environmental Microbiology, 2008, 74(18): 5769-5775.

[1] ZHANG Heng,LIU Hui-yan,PAN Lin,WANG Hong-yan,LI Xiao-fang,WANG Tong,FANG Hai-tian. Research Strategy for Biosynthesis of Gamma Aminobutyric Acid[J]. China Biotechnology, 2021, 41(8): 110-119.
[2] WANG Yi-han,LI Hai-yan,XUE Yong-chang. The Structural Characteristics and Engineering Reconstruction of Flavin-dependent Halogenase[J]. China Biotechnology, 2021, 41(4): 74-80.
[3] HE Wei,ZHU Lei,LIU Xin-ze,AN Xue-li,WAN Xiang-yuan. Research Progress on Maize Genetic Transformation and Commercial Development of Transgenic Maize[J]. China Biotechnology, 2021, 41(12): 13-23.
[4] WU Han-rong,WANG Ying,HUANG Ying-ming,LI Dong-xue,LI Zhi-fei,FANG Zi-han,FAN Lin. Promote the Innovation and Transformation of Biotechnology by Base Platform[J]. China Biotechnology, 2021, 41(12): 141-147.
[5] MENG Xiao-lin,PANG Xi-ming,WANG Jie. Agrobacterium-mediated Transformation and the Functions of Pks in Marine-derived Penicillium oxalicum[J]. China Biotechnology, 2020, 40(9): 11-17.
[6] YANG Li,SHI Xiao-yu,LI Wen-lei,LI Jian,XU Han-mei. Optimization of Electroporation Conditions in Construction of Phage Display Antibody Library[J]. China Biotechnology, 2020, 40(4): 42-48.
[7] Yue-lei FAN,Jiao LU,Da-ming CHEN,Kai-yun MAO. Strategies for Stem Cell Patent Evaluation and Patent Transfer and Transformation[J]. China Biotechnology, 2019, 39(1): 99-106.
[8] Bo-wen CHEN,Hai-long LIU,Yu-fei XIAO,Zi-hai QIN,Ye ZHANG,Xiao-ning ZHANG. Directional Regulation of Lignin Monomer Synthesis in Tobacco by Using COMT Gene and CCoAOMT Gene of Eucalyptus urophylla[J]. China Biotechnology, 2018, 38(3): 24-32.
[9] Zheng-san ZUO,Dong-sheng GUO,Xiao-jun JI,Ping SONG,He HUANG. Polyunsaturated Fatty Acids and Their Derivatives in the Intestinal Tract:a Review[J]. China Biotechnology, 2018, 38(11): 66-75.
[10] Ting AN,Jing JI,Yu-rong WANG,Zhi-gang MA,Gang WANG,Qian LI,Dan YANG,Song-hao ZHANG. Analysis of the Transformation Efficiency and Induced Differentiation of Lilium brownii Scales[J]. China Biotechnology, 2018, 38(1): 25-31.
[11] XIA Hui, LIU Lei, WANG Xiu, SHEN Yan-qiu, GUO Yu-lun, LIANG Dong. Research on Stress-inducible Expression Characteristics of Sorbitol-6- phosphate Dehydrogenase Promoter from Apple[J]. China Biotechnology, 2017, 37(6): 50-55.
[12] SUN Dan, ZHANG Min, XIE Chang-rui, GUO Xiao-wei, XU He-han, GAO Hong-tao, LI Xiao-wei, SUN Tian-xu, LI Hai-yan. Establishment of Genetic Transformation System of Cordyceps militaris using PEG Mediated Method[J]. China Biotechnology, 2017, 37(4): 76-82.
[13] YAO Ren-hui, DONG Zhuo, LI Hui. Biotransformation of Androst-4-en-3,17-dione by Gibberella intermedia C2[J]. China Biotechnology, 2017, 37(3): 73-77.
[14] YU Xiao-chun, MA Shi-liang. Advances in Research of Aspergillus oryzae as a Host of Heterologous Protein Expression[J]. China Biotechnology, 2016, 36(9): 94-100.
[15] ZENG Si-yu, SHI Tian-qiong, SHI Kun, REN Lu-jing, HUANG He, JI Xiao-jun. Establishment and Application of Genetic Motification System for Mortierella alpina[J]. China Biotechnology, 2016, 36(7): 112-116.