Relationship between the Bioactivity of BMP2/7 Heterodimers and its Regulation of CIZ Expression

China Biotechnology

2007, Vol. 27

Issue (9): 14-18 DOI:

Relationship between the Bioactivity of BMP2/7 Heterodimers and its Regulation of CIZ Expression

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Abstract

Abstract BMP2/7 heterodimer has stronger bioactivity than BMP2 homodimer, but the mechanism remains to be unclear. Treating the osteoblast MC3T3-E1 cells with BMP proteins, results of Alkalin phosphatase staining , Alizarin red staining , as well as p3GC2-LUX luciferase assay demonstrated that BMP2/7 heterodimers significantly promoted osteogenic differentiation to a higher extent than BMP2 homodimers. Overexpression of CIZ in MC3T3-E1 significantly inhibited the promotion of ALP and Osteocalcin expression by BMP2/7, and blocked activation of BMP/Smad. BMP protein may induce the expression of CIZ, but effects of BMP2/7 heterodimer is weaker than BMP2 homodimer. It suggested that the effects of BMP2/7 on CIZ expression is related to its bioactivity.

Key words： Bone morphogenetic protein heterodimer osteoblast differentiation CIZ protein

Received: 16 May 2007 Published: 25 September 2007

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Cite this article:

. Relationship between the Bioactivity of BMP2/7 Heterodimers and its Regulation of CIZ Expression. China Biotechnology, 2007, 27(9): 14-18.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I9/14

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