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The Impacts of Feeder Cells on Culture in vitro of Sheep Embryonic Stem Cells |
ZHAO Rui-yuan, LIU Chun-xia, LI Hui-peng, WANG Shen-yuan, ZHOU Huan-min |
College of Life Science, Inner Mongolia Agricultural University; Key Laboratory of Biological Manufacturing Inner Mongolia Auto Region, Hohhot 010018, China |
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Abstract Embryonic stem cell (ESC) research progresses show slowly on many species, especially on livestock. Although sheep ESC research has long been reported, but not yet established stabilize passaged cell lines. On the basis of the establishing system in vitro fertilization and development of sheep, the experiment explored the impact of feeder layers (Feeder) cells on sheep ESC, including processing time of feeder on certain concentration of mitomycin, cell seeding density, cell species, cell passages, and feeder cells freshly prepared and frozen. The aim of the comparative study is to optimize the appropriate feeder cells for the establishment of sheep ESC culture system in vitro. The results showed that the first to the fifth passage of SEF and the first to the third passage MEF and the 1:1 mixture of both cells which were treated by 10 μg/ml mitomycin for 2~2.2 hours could better support the growth of sheep ESC.
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Received: 04 November 2014
Published: 25 February 2015
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[1] Evans M J, Kaufman M H. Establishment in culture of pluripotential cells from mouse embryos. Nature, 1981, 292: 154-156.
[2] Thomson J A, Kalishman J, Golo T G, et al. Isolation of a primate embryonic stem cell line. Proc Natl Acad Sci USA, 1995, 92(17): 7844-7848.
[3] Thomson J A, Kalishman J, Golos T G, et al. Pluripotent cell lines derived from common marmoset (Callithrix jacchus) blastocysts. Biol Reprod, 1996, 55:254-259.
[4] Thomson J A, Itskovits-Eldor J, Shapiro S S, et al. Embryonic stem cell lines derived from human blastocysts. Science, 1998, 282: 1145-1147.
[5] Zhao YunCheng, Jiapeng Lin, Liqin Wang, et al. Derivation and characterization of ovine embryonic stem-like cell lines in semi-defined medium without feeder cells. J Exp Zool, 2011, 315: 639-648.
[6] 周川,赵云程,林嘉鹏,等.绵羊类胚胎干细胞长期培养中增殖、凋亡、多能性相关基因的表达动力学.中国生物化学与分子生物学报, 2012, 28(6): 574-579. Zhou C, Zhao Y C, Lin J P, et al. The proliferation, apoptosis, and expression kinetics of pluripotency genes of sheep embryonic stem-like cells in long-term cultures. Chinese Journal of Biochemistry and Molecular Biology, 2012, 28 (6): 574-579.
[7] 薛庆善.体外培养的原理与技术.北京:科学出版社,2001 Xue Q S. Principles and Technology in vitro Culture, Beijing: Science Press, 2001.
[8] Carpenter M K, Rosler E S, Fisk G J. Properties of four human embryonic stem cell lines maintained in a feeder-free culture system. Dev Dyn, 2004, 229(2): 243-258.
[9] Rosler E S, Fisk G J, Ares X. Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dyn, 2004, 229(2): 259-274.
[10] Amit M, Shariki C, Margulets V. Feeder layer and serum-free culture of human embryonic stem cells. Biol Reprod, 2004, 70(3): 837-845.
[11] Stojek R M, Reed M A, Hoover J L. A method for cultivating morphologically undifferentiated embryonic stem cell from porcine blastocysts. Theriogenology, 1990, 33(4):912-913.
[12] Richards M, Fong C Y, Chan W K. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol, 2002, 20(9): 933-936.
[13] 任卫青,陈亮,和小娥,等.绵羊类胚胎干细胞的分离与克隆.中国兽医学报, 2012,32(5):788-792. Ren W Q,Chen L,He X E,et al.The isolation and cloning of sheep embryonic stem-like cells. China J Vet Sci, 2012,32(5):788-792.
[14] 赖良学. 影响体外培养兔胚发育和兔类ES细胞分离的若干因素.中国兽医学报,1996,16(1):16-21. Lai X L. Factors of influencing the embryo development and separation of its ES cell from in vitro cultured rabbits embryo. China J Vet Sci,1996,16(1):16-21.
[15] 刘羿羿. 山羊类胚胎干细胞和PGCs 的培养与鉴定. 呼和浩特:内蒙古农业大学,2010. Liu Y Y. Culture and Identification of Goat Embryonic Stem Like Cells and PGCs. Huhhot:Inner Mongolia Agricultural University, 2010.
[16] Dharmendra Kumar, Anand T, Singh K P. Derivation of buffalo embryonic stem-like cells from in vitro produced blastocysts on homologous and heterologous feeder cells. J Assist Reprod Genet, 2011, 28: 679-688.
[17] Jozefczuk J, Drews K, Adjaye J. Preparation of mouse embryonic fibroblast cells suitable for culturing human embryonic and induced pluripotent stem cells. Journal of Visualized Experiments, 2012, 64:e3854.
[18] Mitsui K, Tokuzawa Y, Itoh H, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell, 2003, 113:631-642.
[19] 夏金超, 陈凌. Nanog基因及其传导通路在保持胚胎干细胞多能性中的作用.中华临床医师杂志,2012,8:2156-2158. Xia J C, Chen L. Function of nanog and its pathway in maintaining the pluripotency of embryonic stem cells. Chin J Clinicians, 2012,8:2156-2158.
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